EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Specific interaction between EF-G and RRF and its implication for GTP-dependent ribosome splitting into subunits.
ジャーナル・号・ページ	J Mol Biol, Vol. 374, Issue 5, Page 1345-1358, Year 2007
掲載日	2007年12月14日
著者	Ning Gao / Andrey V Zavialov / Måns Ehrenberg / Joachim Frank /
PubMed 要旨	After termination of protein synthesis, the bacterial ribosome is split into its 30S and 50S subunits by the action of ribosome recycling factor (RRF) and elongation factor G (EF-G) in a guanosine 5'- ...After termination of protein synthesis, the bacterial ribosome is split into its 30S and 50S subunits by the action of ribosome recycling factor (RRF) and elongation factor G (EF-G) in a guanosine 5'-triphosphate (GTP)-hydrolysis-dependent manner. Based on a previous cryo-electron microscopy study of ribosomal complexes, we have proposed that the binding of EF-G to an RRF-containing posttermination ribosome triggers an interdomain rotation of RRF, which destabilizes two strong intersubunit bridges (B2a and B3) and, ultimately, separates the two subunits. Here, we present a 9-A (Fourier shell correlation cutoff of 0.5) cryo-electron microscopy map of a 50S x EF-G x guanosine 5'-[(betagamma)-imido]triphosphate x RRF complex and a quasi-atomic model derived from it, showing the interaction between EF-G and RRF on the 50S subunit in the presence of the noncleavable GTP analogue guanosine 5'-[(betagamma)-imido]triphosphate. The detailed information in this model and a comparative analysis of EF-G structures in various nucleotide- and ribosome-bound states show how rotation of the RRF head domain may be triggered by various domains of EF-G. For validation of our structural model, all known mutations in EF-G and RRF that relate to ribosome recycling have been taken into account. More importantly, our results indicate a substantial conformational change in the Switch I region of EF-G, suggesting that a conformational signal transduction mechanism, similar to that employed in transfer RNA translocation on the ribosome by EF-G, translates a large-scale movement of EF-G's domain IV, induced by GTP hydrolysis, into the domain rotation of RRF that eventually splits the ribosome into subunits.
リンク	J Mol Biol / PubMed:17996252 / PubMed Central
手法	EM (単粒子)
解像度	9.1 Å
構造データ	1430 2rdo EMDB-1430: Specific interaction between EF-G and RRF and its implication for GTP-dependent ribosome splitting into subunits. PDB-2rdo: 50S subunit with EF-G(GDPNP) and RRF bound 手法: EM (単粒子) / 解像度: 9.1 Å
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME (リボソーム) / elongation factor G (EF-G) / EF-G (EF-G) / RRF / ribosome recycling factor / GDPNP / 50S subunit / cryo-EM (低温電子顕微鏡法) / Real-space refinement / Ribonucleoprotein (核タンパク質) / Ribosomal protein / RNA-binding / rRNA-binding / Methylation (メチル化) / Antibiotic resistance (抗微生物薬耐性) / Repressor (リプレッサー) / Transcription (転写 (生物学)) / Transcription regulation / Transcription termination / Translation regulation / tRNA-binding / Phosphoprotein / Metal-binding / GTP-binding / Nucleotide-binding / Protein biosynthesis (タンパク質生合成)