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-Structure paper
タイトル | Structural aspects of RbfA action during small ribosomal subunit assembly. |
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ジャーナル・号・ページ | Mol Cell, Vol. 28, Issue 3, Page 434-445, Year 2007 |
掲載日 | 2007年11月9日 |
著者 | Partha P Datta / Daniel N Wilson / Masahito Kawazoe / Neil K Swami / Tatsuya Kaminishi / Manjuli R Sharma / Timothy M Booth / Chie Takemoto / Paola Fucini / Shigeyuki Yokoyama / Rajendra K Agrawal / |
PubMed 要旨 | Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ...Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock. |
リンク | Mol Cell / PubMed:17996707 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.84 - 12.5 Å |
構造データ | EMDB-1413: Structural aspects of RbfA action during small ribosomal subunit assembly. PDB-2dyj: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | RIBOSOMAL PROTEIN / 16S rRNA processing / 17S RNA / KH domain / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI / RNA BINDING PROTEIN / helix-kink-helix / rRNA processing / RIBOSOMAL PROTEIN/RNA / 30S RIBOSOME MATURATION PROTEIN RbfA / COLD SHOCK RESPONSE PROTEIN RbfA / 30S-RbfA COMPLEX / RbfA BINDING SITE ON THE 30S / Ribonucleoprotein / RNA-binding / rRNA-binding / tRNA-binding / Antibiotic resistance / RIBOSOMAL PROTEIN-RNA COMPLEX |