EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular Architecture of Contactin-associated Protein-like 2 (CNTNAP2) and Its Interaction with Contactin 2 (CNTN2).
ジャーナル・号・ページ	J Biol Chem, Vol. 291, Issue 46, Page 24133-24147, Year 2016
掲載日	2016年11月11日
著者	Zhuoyang Lu / M V V V Sekhar Reddy / Jianfang Liu / Ana Kalichava / Jiankang Liu / Lei Zhang / Fang Chen / Yun Wang / Luis Marcelo F Holthauzen / Mark A White / Suchithra Seshadrinathan / Xiaoying Zhong / Gang Ren / Gabby Rudenko /
PubMed 要旨	Contactin-associated protein-like 2 (CNTNAP2) is a large multidomain neuronal adhesion molecule implicated in a number of neurological disorders, including epilepsy, schizophrenia, autism spectrum ...Contactin-associated protein-like 2 (CNTNAP2) is a large multidomain neuronal adhesion molecule implicated in a number of neurological disorders, including epilepsy, schizophrenia, autism spectrum disorder, intellectual disability, and language delay. We reveal here by electron microscopy that the architecture of CNTNAP2 is composed of a large, medium, and small lobe that flex with respect to each other. Using epitope labeling and fragments, we assign the F58C, L1, and L2 domains to the large lobe, the FBG and L3 domains to the middle lobe, and the L4 domain to the small lobe of the CNTNAP2 molecular envelope. Our data reveal that CNTNAP2 has a very different architecture compared with neurexin 1α, a fellow member of the neurexin superfamily and a prototype, suggesting that CNTNAP2 uses a different strategy to integrate into the synaptic protein network. We show that the ectodomains of CNTNAP2 and contactin 2 (CNTN2) bind directly and specifically, with low nanomolar affinity. We show further that mutations in CNTNAP2 implicated in autism spectrum disorder are not segregated but are distributed over the whole ectodomain. The molecular shape and dimensions of CNTNAP2 place constraints on how CNTNAP2 integrates in the cleft of axo-glial and neuronal contact sites and how it functions as an organizing and adhesive molecule.
リンク	J Biol Chem / PubMed:27621318 / PubMed Central
手法	EM (トモグラフィー) / EM (単粒子)
解像度	12.0 - 20.0 Å
構造データ	EMDB-6717: Contactin-2 reconstructed by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 20.0 Å EMDB-9543: IPET 3D reconstruction of an single CNTNAP2-antibody complex: conformation #1 手法: EM (トモグラフィー) / 解像度: 16.3 Å EMDB-9544: IPET 3D reconstruction of a single CNTNAP2-antibody complex: conformation #2 手法: EM (トモグラフィー) / 解像度: 16.3 Å EMDB-9545: Single particle reconstruction of CNTNAP2: conformation #7 手法: EM (単粒子) / 解像度: 15.6 Å EMDB-9546: Single particle reconstruction of CNTNAP2: conformation #8 手法: EM (単粒子) / 解像度: 15.7 Å EMDB-9547: IPET 3D reconstruction of a single CNTNAP2 - 1.8 nm nanogold complex 手法: EM (トモグラフィー) / 解像度: 14.6 Å EMDB-9548: IPET 3D reconstruction of a single CNTNAP2 - 5 nm nanogold complex 手法: EM (トモグラフィー) / 解像度: 12.0 Å EMDB-9549: IPET 3D reconstruction of a single CNTNAP2-C2 domain 手法: EM (トモグラフィー) / 解像度: 20.0 Å EMDB-9550: Single particle reconstruction of CNTNAP2: conformation #6 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-9551: Single particle reconstruction of CNTNAP2: conformation #5 手法: EM (単粒子) / 解像度: 16.4 Å EMDB-9552: Single particle reconstruction of CNTNAP2: conformation #4 手法: EM (単粒子) / 解像度: 13.7 Å EMDB-9553: Single particle reconstruction of CNTNAP2: conformation #3 手法: EM (単粒子) / 解像度: 15.9 Å EMDB-9554: Single particle reconstruction of CNTNAP2: conformation #2 手法: EM (単粒子) / 解像度: 15.7 Å EMDB-9555: Single particle reconstruction of CNTNAP2: conformation #1 手法: EM (単粒子) / 解像度: 15.3 Å EMDB-9556: IPET 3D reconstruction of a single CNTNAP2: conformation #8 手法: EM (トモグラフィー) / 解像度: 16.9 Å EMDB-9557: IPET 3D reconstruction of a single CNTNAP2: conformation #7 手法: EM (トモグラフィー) / 解像度: 17.0 Å EMDB-9558: IPET 3D reconstruction of a single CNTNAP2: conformation #6 手法: EM (トモグラフィー) / 解像度: 15.6 Å EMDB-9559: IPET 3D reconstruction of a single CNTNAP2: conformation #5 手法: EM (トモグラフィー) / 解像度: 16.0 Å EMDB-9560: IPET 3D reconstruction of a single CNTNAP2: conformation #4 手法: EM (トモグラフィー) / 解像度: 16.5 Å EMDB-9561: IPET 3D reconstruction of a single CNTNAP2: conformation #3 手法: EM (トモグラフィー) / 解像度: 17.5 Å EMDB-9562: IPET 3D reconstruction of a single CNTNAP2: conformation #2 手法: EM (トモグラフィー) / 解像度: 18.9 Å EMDB-9563: IPET 3D reconstruction of a single CNTNAP2: conformation #1 手法: EM (トモグラフィー) / 解像度: 16.3 Å
由来	Homo sapiens (ヒト)