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-Structure paper
タイトル | Alternative Mode of E-Site tRNA Binding in the Presence of a Downstream mRNA Stem Loop at the Entrance Channel. |
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ジャーナル・号・ページ | Structure, Vol. 26, Issue 3, Page 437-445.e3, Year 2018 |
掲載日 | 2018年3月6日 |
著者 | Yan Zhang / Samuel Hong / Ajchareeya Ruangprasert / Georgios Skiniotis / Christine M Dunham / |
PubMed 要旨 | Structured mRNAs positioned downstream of the ribosomal decoding center alter gene expression by slowing protein synthesis. Here, we solved the cryo-EM structure of the bacterial ribosome bound to an ...Structured mRNAs positioned downstream of the ribosomal decoding center alter gene expression by slowing protein synthesis. Here, we solved the cryo-EM structure of the bacterial ribosome bound to an mRNA containing a 3' stem loop that regulates translation. Unexpectedly, the E-site tRNA adopts two distinct orientations. In the first structure, normal interactions with the 50S and 30S E site are observed. However, in the second structure, although the E-site tRNA makes normal interactions with the 50S E site, its anticodon stem loop moves ∼54 Å away from the 30S E site to interact with the 30S head domain and 50S uL5. This position of the E-site tRNA causes the uL1 stalk to adopt a more open conformation that likely represents an intermediate state during E-site tRNA dissociation. These results suggest that structured mRNAs at the entrance channel restrict 30S subunit movement required during translation to slow E-site tRNA dissociation. |
リンク | Structure / PubMed:29456023 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.2 - 3.5 Å |
構造データ | |
化合物 | ChemComp-ZN: ChemComp-SF4: |
由来 |
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キーワード | RIBOSOME / 70S ribosome / dnaX / stem-loop / structured mRNA / -1 programmed frameshifting / E site |