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-Structure paper
タイトル | A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM. |
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ジャーナル・号・ページ | Structure, Vol. 25, Issue 4, Page 663-670.e3, Year 2017 |
掲載日 | 2017年4月4日 |
![]() | Xiangsong Feng / Ziao Fu / Sandip Kaledhonkar / Yuan Jia / Binita Shah / Amy Jin / Zheng Liu / Ming Sun / Bo Chen / Robert A Grassucci / Yukun Ren / Hongyuan Jiang / Joachim Frank / Qiao Lin / ![]() ![]() |
PubMed 要旨 | We describe a spraying-plunging method for preparing cryoelectron microscopy (cryo-EM) grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device. The new ...We describe a spraying-plunging method for preparing cryoelectron microscopy (cryo-EM) grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device. The new polydimethylsiloxane (PDMS)-based sprayer was tested with apoferritin. We demonstrate that the structure can be solved to high resolution with this method of sample preparation. Besides replacing the conventional pipetting-blotting-plunging method, one of many potential applications of the new sprayer is in time-resolved cryo-EM, as part of a PDMS-based microfluidic reaction channel to study short-lived intermediates on the timescale of 10-1,000 ms. |
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手法 | EM (単粒子) |
解像度 | 3.0 Å |
構造データ | ![]() EMDB-8428: |
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