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-Structure paper
タイトル | Visualizing the molecular sociology at the HeLa cell nuclear periphery. |
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ジャーナル・号・ページ | Science, Vol. 351, Issue 6276, Page 969-972, Year 2016 |
掲載日 | 2016年2月26日 |
著者 | Julia Mahamid / Stefan Pfeffer / Miroslava Schaffer / Elizabeth Villa / Radostin Danev / Luis Kuhn Cuellar / Friedrich Förster / Anthony A Hyman / Jürgen M Plitzko / Wolfgang Baumeister / |
PubMed 要旨 | The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots ...The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness. |
リンク | Science / PubMed:26917770 |
手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
解像度 | 28.0 - 92.0 Å |
構造データ | EMDB-11992: EMDB-8054: EMDB-8055: EMDB-8056: EMDB-8057: |
由来 |
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