EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Separating the effects of nucleotide and EB binding on microtubule structure.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 115, Issue 27, Page E6191-E6200, Year 2018
掲載日	2018年7月3日
著者	Rui Zhang / Benjamin LaFrance / Eva Nogales /
PubMed 要旨	Microtubules (MTs) are polymers assembled from αβ-tubulin heterodimers that display the hallmark behavior of dynamic instability. MT dynamics are driven by GTP hydrolysis within the MT lattice, and ...Microtubules (MTs) are polymers assembled from αβ-tubulin heterodimers that display the hallmark behavior of dynamic instability. MT dynamics are driven by GTP hydrolysis within the MT lattice, and are highly regulated by a number of MT-associated proteins (MAPs). How MAPs affect MTs is still not fully understood, partly due to a lack of high-resolution structural data on undecorated MTs, which need to serve as a baseline for further comparisons. Here we report three structures of MTs in different nucleotide states (GMPCPP, GDP, and GTPγS) at near-atomic resolution and in the absence of any binding proteins. These structures allowed us to differentiate the effects of nucleotide state versus MAP binding on MT structure. Kinesin binding has a small effect on the extended, GMPCPP-bound lattice, but hardly affects the compacted GDP-MT lattice, while binding of end-binding (EB) proteins can induce lattice compaction (together with lattice twist) in MTs that were initially in an extended and more stable state. We propose a MT lattice-centric model in which the MT lattice serves as a platform that integrates internal tubulin signals, such as nucleotide state, with outside signals, such as binding of MAPs or mechanical forces, resulting in global lattice rearrangements that in turn affect the affinity of other MT partners and result in the exquisite regulation of MT dynamics.
リンク	Proc Natl Acad Sci U S A / PubMed:29915050 / PubMed Central
手法	EM (らせん対称)
解像度	3.1 - 4.3 Å
構造データ	7973 6dpu EMDB-7973, PDB-6dpu: Undecorated GMPCPP microtubule 手法: EM (らせん対称) / 解像度: 3.1 Å 7974 6dpv EMDB-7974, PDB-6dpv: Undecorated GDP microtubule 手法: EM (らせん対称) / 解像度: 3.3 Å 7975 6dpw EMDB-7975, PDB-6dpw: Undecorated GTPgammaS microtubule 手法: EM (らせん対称) / 解像度: 3.5 Å EMDB-7976: Preformed GMPCPP microtubule washed with EB3, class 1 手法: EM (らせん対称) / 解像度: 4.3 Å EMDB-7977: Preformed GMPCPP microtubule washed with EB3, class 2 手法: EM (らせん対称) / 解像度: 4.1 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-GSP*: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S
由来	sus scrofa (ブタ) Homo sapiens (ヒト)
キーワード	CELL CYCLE / microtubule / cytoskeleton / GMPCPP / seam / dynamic / GTPgammaS