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-Structure paper
タイトル | Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel. |
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ジャーナル・号・ページ | Nat Commun, Vol. 10, Issue 1, Page 3740, Year 2019 |
掲載日 | 2019年8月20日 |
著者 | Ying Yin / Mengyu Wu / Allen L Hsu / William F Borschel / Mario J Borgnia / Gabriel C Lander / Seok-Yong Lee / |
PubMed 要旨 | The transient receptor potential melastatin 2 (TRPM2) channel plays a key role in redox sensation in many cell types. Channel activation requires binding of both ADP-ribose (ADPR) and Ca. The ...The transient receptor potential melastatin 2 (TRPM2) channel plays a key role in redox sensation in many cell types. Channel activation requires binding of both ADP-ribose (ADPR) and Ca. The recently published TRPM2 structures from Danio rerio in the ligand-free and the ADPR/Ca-bound conditions represent the channel in closed and open states, which uncovered substantial tertiary and quaternary conformational rearrangements. However, it is unclear how these rearrangements are achieved within the tetrameric channel during channel gating. Here we report the cryo-electron microscopy structures of Danio rerio TRPM2 in the absence of ligands, in complex with Ca alone, and with both ADPR and Ca, resolved to ~4.3 Å, ~3.8 Å, and ~4.2 Å, respectively. In contrast to the published results, our studies capture ligand-bound TRPM2 structures in two-fold symmetric intermediate states, offering a glimpse of the structural transitions that bridge the closed and open conformations. |
リンク | Nat Commun / PubMed:31431622 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.8 - 4.5 Å |
構造データ | EMDB-20367, PDB-6pkv: EMDB-20368, PDB-6pkw: EMDB-20369, PDB-6pkx: |
化合物 | ChemComp-CA: ChemComp-AR6: |
由来 |
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キーワード | TRANSPORT PROTEIN / warmth sensor / redox sensor / calcium-permeable ion channel / ion channel / TRP channel / TRPM channel / ADP-ribose |