EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Human GlyRα2 pore dynamics in gating and inhibition.
ジャーナル・号・ページ	Structure, Year 2026
掲載日	2026年3月18日
著者	Emily Klemm / Eric Gibbs / Madeleine Stauffer / Devesh Mohapatra / Chloe Meyer / Sudha Chakrapani /
PubMed 要旨	Glycine receptors (GlyRs) mediate inhibitory neurotransmission in the central nervous system. The GlyRα2 subtype contributes to critical neural circuitry in early neurodevelopment and is also found ...Glycine receptors (GlyRs) mediate inhibitory neurotransmission in the central nervous system. The GlyRα2 subtype contributes to critical neural circuitry in early neurodevelopment and is also found in adults. GlyRα2 dysfunctions are implicated in neurodevelopmental disorders, including autism, epilepsy, and cognitive delays. GlyRα2 functional properties and pharmacology are distinct from GlyRα1, but the structural basis for these differences remains poorly defined. Here, we report cryo-electron microscopy structures of full-length, human GlyRα2 reconstituted in peptidiscs captured in multiple conformational states. In addition to symmetric resting and desensitized states, we resolved an asymmetric open state, previously observed only in heteromeric GlyRs. This suggests that asymmetry is intrinsic to GlyRα2, independent of β-subunit incorporation. Furthermore, we identified distinct conformations of GlyRα2 with the pore-blocker picrotoxin, providing new insights into allosteric interactions. These findings uncover the structural basis of GlyRα2 function, providing a foundation for understanding its role in development and in GlyRα2-associated disorders.
リンク	Structure / PubMed:41856109 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.6 Å
構造データ	72665 9y7p EMDB-72665, PDB-9y7p: Homomeric Glycine Receptor alpha2 with 1 mM Glycine in a Desensitized State 手法: EM (単粒子) / 解像度: 2.5 Å 72668 9y7w EMDB-72668, PDB-9y7w: Homomeric Glycine Receptor alpha2 with 1 mM Glycine in an Open State 手法: EM (単粒子) / 解像度: 3.19 Å 72674 9y7x EMDB-72674, PDB-9y7x: Homomeric Glycine Receptor alpha2 with 0.1 mM Glycine in an Open State 手法: EM (単粒子) / 解像度: 3.55 Å 72675 9y7z EMDB-72675, PDB-9y7z: Homomeric Glycine Receptor alpha2 with 0.1 mM Glycine in a Desensitized State 手法: EM (単粒子) / 解像度: 2.78 Å 72676 9y80 EMDB-72676, PDB-9y80: Homomeric Glycine Receptor alpha2 with PTX in a Desensitized State 手法: EM (単粒子) / 解像度: 3.55 Å 72683 9y8z EMDB-72683, PDB-9y8z: Homomeric Glycine Receptor alpha2 with 0.1 mM Glycine in an Apo State 手法: EM (単粒子) / 解像度: 2.5 Å 72688 9y94 EMDB-72688, PDB-9y94: Homomeric Glycine Receptor alpha2 with PTX in an Open State 手法: EM (単粒子) / 解像度: 3.6 Å 72689 9y95 EMDB-72689, PDB-9y95: Homomeric Glycine Receptor alpha2 with PTX in an Apo State 手法: EM (単粒子) / 解像度: 3.45 Å 72690 9y96 EMDB-72690, PDB-9y96: Homomeric Glycine Receptor alpha2 with 1 mM Glycine in a Closed State 手法: EM (単粒子) / 解像度: 3.36 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-GLY: GLYCINE ChemComp-PX4: 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DMPC, リン脂質YM ChemComp-RI5: (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c / toxinYM ChemComp-CLR: CHOLESTEROL
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / homopentamer / ion channel / ligand gated ion channel