EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the activation of the chicken ROS1 receptor by the NEL/NICOL ligand complex.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年2月24日
著者	Weidong An / Xuewu Zhang / Xiao-Chen Bai /
PubMed 要旨	The receptor tyrosine kinase ROS1 plays essential roles in cell growth and sperm maturation, yet its activation mechanism has remained poorly understood. Here, we report high-resolution cryo-electron ...The receptor tyrosine kinase ROS1 plays essential roles in cell growth and sperm maturation, yet its activation mechanism has remained poorly understood. Here, we report high-resolution cryo-electron microscopy (cryo-EM) structures of chicken ROS1 in its ligand-free form, in complex with its ligand NEL, and with the ligand/co-ligand complex NEL/NICOL. Unliganded ROS1 adopts an arc-shaped conformation. The interaction between NEL and ROS1 is mediated by the VWC2 domain of NEL and the β1 domain of ROS1. Binding of NICOL to the coiled-coil domain of NEL stabilizes NEL into a batwing-shaped asymmetric dimer, which can recruit only one ROS1 molecule due to steric hindrance. Structural analyses and biochemical results suggest that the 2:1 NEL/NICOL complexes further oligomerize through LamG-VWC4 domain interactions, facilitating the clustering of multiple ROS1 for its activation. Functional assays confirm that both NICOL and the multimerization of NEL/NICOL complexes are required for robust ROS1 signaling. Our findings establish NICOL as a critical co-ligand for ROS1 and suggest a distinct ligand-driven oligomerization mechanism for ROS1 activation.
リンク	Nat Commun / PubMed:41735298 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 4.2 Å
構造データ	71042 9oyz EMDB-71042, PDB-9oyz: Cryo-EM structure of chicken ROS1 in apo-state. Cryo-EM refinement is focused on the "head" region of chicken ROS1. 手法: EM (単粒子) / 解像度: 2.9 Å 71047 9oz1 EMDB-71047, PDB-9oz1: Cryo-EM structure of chicken ROS1 in apo-state. Cryo-EM refinement is focused on the "leg" region of chicken ROS1. 手法: EM (単粒子) / 解像度: 3.6 Å 71049 9oz6 EMDB-71049, PDB-9oz6: Cryo-EM structure of 1:1 chicken ROS1 and chicken NEL complex. 手法: EM (単粒子) / 解像度: 2.7 Å 71051 9oz8 EMDB-71051, PDB-9oz8: Cryo-EM structure of chicken NEL dimer bound with one human NICOL. 手法: EM (単粒子) / 解像度: 4.1 Å 71057 9ozc EMDB-71057, PDB-9ozc: Cryo-EM structure of 1:2:1 ROS1/NEL/NICOL holo-complex, conformation 1. 手法: EM (単粒子) / 解像度: 4.0 Å 71058 9ozh EMDB-71058, PDB-9ozh: Cryo-EM structure of 1:2:1 ROS1/NEL/NICOL holo-complex, conformation 2. 手法: EM (単粒子) / 解像度: 4.2 Å 71059 9ozi EMDB-71059, PDB-9ozi: Cryo-EM structure of chicken ROS1 in apo-state. This is the complete map and model. 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose
由来	gallus gallus (ニワトリ) homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN / ROS1 / SIGNALING PROTEIN / NEL