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-Structure paper
| タイトル | Heterogeneity of radial spoke components in Tetrahymena cilia. |
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| ジャーナル・号・ページ | Cell Mol Life Sci, Vol. 82, Issue 1, Page 329, Year 2025 |
| 掲載日 | 2025年8月31日 |
著者 | Marta Bicka / Avrin Ghanaeian / Corbin Black / Ewa Joachimiak / Anna Osinka / Sumita Majhi / Anna Konopka / Ewa Bulska / Khanh Huy Bui / Dorota Wloga / ![]() |
| PubMed 要旨 | Radial spokes, RS1, RS2, and RS3, are T-shaped, multiprotein complexes that transmit regulatory signals from the central apparatus to outer doublet complexes, including dynein arms. Radial spokes, ...Radial spokes, RS1, RS2, and RS3, are T-shaped, multiprotein complexes that transmit regulatory signals from the central apparatus to outer doublet complexes, including dynein arms. Radial spokes, especially RS3, differ in their morphology, protein composition, and RS base-docked IDAs. Spokes' defects alter cilia beating frequency, waveform, and amplitude, leading, in humans, to primary ciliary dyskinesia and male infertility. The role of the particular spokes remains unclear. Ciliate Tetrahymena thermophila has three RSP3 paralogs and two or three paralogs of some other RSPs. Using multiple complementary approaches, we demonstrated that Tetrahymena forms RS1 and RS2 subtypes, each with a core composed of various RSP3 paralogs and one type of RSP3-less RS3. We elucidated proteomes of RS1 and RS2 subtypes, RS3, and identified novel RS proteins, including enzymatic proteins involved in local regulation of ADP/ATP levels, the guanylate nucleotide pathway, and protein phosphorylation, whose presence further diversifies RSs' properties and likely functions. |
リンク | Cell Mol Life Sci / PubMed:40886204 / PubMed Central |
| 手法 | EM (サブトモグラム平均) |
| 解像度 | 17.16 - 33.13 Å |
| 構造データ | ![]() EMDB-70169: Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3A-KO Tetrahymena thermophila ![]() EMDB-70171: Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3C-KO Tetrahymena thermophila ![]() EMDB-70172: Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3A-HA-BCCP Tetrahymena thermophila |
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