EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly.
ジャーナル・号・ページ	Elife, Vol. 7, Year 2018
掲載日	2018年5月9日
著者	Hiroshi Yamaguchi / Toshiyuki Oda / Masahide Kikkawa / Hiroyuki Takeda /
PubMed 要旨	Construction of motile cilia/flagella requires cytoplasmic preassembly of axonemal dyneins before transport into cilia. Axonemal dyneins have various subtypes, but the roles of each dynein subtype ...Construction of motile cilia/flagella requires cytoplasmic preassembly of axonemal dyneins before transport into cilia. Axonemal dyneins have various subtypes, but the roles of each dynein subtype and their assembly processes remain elusive in vertebrates. The PIH protein family, consisting of four members, has been implicated in the assembly of different dynein subtypes, although evidence for this idea is sparse. Here, we established zebrafish mutants of all four PIH-protein genes: , , , and , and analyzed the structures of axonemal dyneins in mutant spermatozoa by cryo-electron tomography. Mutations caused the loss of specific dynein subtypes, which was correlated with abnormal sperm motility. We also found organ-specific compositions of dynein subtypes, which could explain the severe motility defects of mutant Kupffer's vesicle cilia. Our data demonstrate that all vertebrate PIH proteins are differently required for cilia/flagella motions and the assembly of axonemal dyneins, assigning specific dynein subtypes to each PIH protein.
リンク	Elife / PubMed:29741156 / PubMed Central
手法	EM (トモグラフィー)
解像度	41.7 - 45.1 Å
構造データ	EMDB-6954: Doublet microtubule of zebrafish sperm axoneme, WT 手法: EM (トモグラフィー) / 解像度: 42.5 Å EMDB-6955: Doublet microtubule of zebrafish sperm axoneme, pih1d1_null mutant 手法: EM (トモグラフィー) / 解像度: 45.0 Å EMDB-6956: Doublet microtubule of zebrafish sperm axoneme, pih1d2_null mutant 手法: EM (トモグラフィー) / 解像度: 45.1 Å EMDB-6957: Doublet microtubule of zebrafish sperm axoneme, ktu_null mutant 手法: EM (トモグラフィー) / 解像度: 43.4 Å EMDB-6958: Doublet microtubule of zebrafish sperm axoneme, twister_null mutant 手法: EM (トモグラフィー) / 解像度: 43.7 Å EMDB-6959: Doublet microtubule of zebrafish sperm axoneme, pih1d2_null and ktu_null double_mutant, +OAD 手法: EM (トモグラフィー) / 解像度: 43.4 Å EMDB-6960: Doublet microtubule of zebrafish sperm axoneme, pih1d2_null and ktu_null double_mutant, -OAD 手法: EM (トモグラフィー) / 解像度: 41.7 Å
由来	Danio rerio (ゼブラフィッシュ)