EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM reveals mechanisms of natural RNA multivalency.
ジャーナル・号・ページ	Science, Page eadv3451, Year 2025
掲載日	2025年3月13日
著者	Liu Wang / Jiahao Xie / Tao Gong / Hao Wu / Yifan Tu / Xin Peng / Sitong Shang / Xinyu Jia / Haiyun Ma / Jian Zou / Sheng Xu / Xin Zheng / Dong Zhang / Yang Liu / Chong Zhang / Yongbo Luo / Zirui Huang / Bin Shao / Binwu Ying / Yu Cheng / Yingqiang Guo / Ying Lai / Dingming Huang / Jianquan Liu / Yuquan Wei / Siqi Sun / Xuedong Zhou / Zhaoming Su /
PubMed 要旨	Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have been rarely ...Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have been rarely reported. Comparative genomics analysis has identified RNA families containing hundreds of sequences that adopt conserved secondary structures and likely fold into complex three-dimensional (3D) structures. We use cryo-electron microscopy (cryo-EM) to determine structures from four RNA families, including ARRPOF and OLE forming dimers, and ROOL and GOLLD forming hexameric, octameric and dodecameric nanostructures, at 2.6 to 4.6 Å resolutions. These homo-oligomeric assemblies reveal a plethora of structural motifs that contribute to RNA multivalency, including kissing loop, palindromic base-pairing, A-stacking, metal ion coordination, pseudoknot and minor-groove interactions. These results provide the molecular basis of intermolecular interactions driving RNA multivalency with potential functional relevance.
リンク	Science / PubMed:40080543
手法	EM (単粒子)
解像度	2.6 - 6.61 Å
構造データ	60850 9isv EMDB-60850, PDB-9isv: Enterococcus faecalis ROOL RNA monomer 手法: EM (単粒子) / 解像度: 3.07 Å 61123 9j3r EMDB-61123: Enterococcus faecalis ncRNA tetramer PDB-9j3r: Enterococcus faecalis ROOL RNA tetramer 手法: EM (単粒子) / 解像度: 4.72 Å 61125 9j3t EMDB-61125: Enterococcus faecalis ncRNA octamer PDB-9j3t: Enterococcus faecalis ROOL RNA octamer 手法: EM (単粒子) / 解像度: 3.8 Å 61189 9j6y EMDB-61189: lactobacillus salivarius ncRNA hexamer PDB-9j6y: Lactobacillus salivarius ROOL RNA hexamer 手法: EM (単粒子) / 解像度: 3.25 Å 62487 9kph EMDB-62487: Fusobacterium ncRNA dimer PDB-9kph: Fusobacterium nucleatum ARRPOF RNA dimer conformation 2 手法: EM (単粒子) / 解像度: 3.93 Å 62489 9kpo EMDB-62489: Fusobacterium ncRNA dimer2 PDB-9kpo: Fusobacterium nucleatum ARRPOF RNA dimer conformation 1 手法: EM (単粒子) / 解像度: 3.98 Å 62721 9l0r EMDB-62721: Streptococcus agalactiae ncRNA decocamer PDB-9l0r: Streptococcus agalactiae GOLLD RNA dodecamer 手法: EM (単粒子) / 解像度: 4.72 Å EMDB-62724: Streptococcus agalactiae GOLLD RNA 3' domain dodecamer 手法: EM (単粒子) / 解像度: 6.61 Å 62991 9lcr EMDB-62991: Clostridium botulinum ncRNA homo-dimer PDB-9lcr: Clostridium botulinum OLE RNA dimer 手法: EM (単粒子) / 解像度: 2.6 Å 63218 9lmf EMDB-63218, PDB-9lmf: Streptococcus agalactiae GOLLD RNA 3' domain decamer 手法: EM (単粒子) / 解像度: 3.93 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	enterococcus faecalis (乳酸球菌) enterococcus faecalis jh1 (乳酸球菌) ligilactobacillus salivarius dsm 20555 = atcc 11741 (バクテリア) fusobacterium nucleatum (バクテリア) streptococcus agalactiae (バクテリア) clostridium botulinum a str. atcc 19397 (ボツリヌス菌)
キーワード	RNA / CryoEM / ncRNA