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-Structure paper
タイトル | Cryo-EM structure of the human glucose transporter GLUT7. |
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ジャーナル・号・ページ | Biochem Biophys Res Commun, Vol. 738, Page 150544, Year 2024 |
掲載日 | 2024年8月13日 |
著者 | Sang Soo Lee / Subin Kim / Mi Sun Jin / |
PubMed 要旨 | GLUT7 is a Class II glucose transporter predominantly expressed at the apical membrane of enterocytes in the small intestine. Here, we report the cryo-EM structure of nanodisc-reconstituted human ...GLUT7 is a Class II glucose transporter predominantly expressed at the apical membrane of enterocytes in the small intestine. Here, we report the cryo-EM structure of nanodisc-reconstituted human GLUT7 in the apo state at 3.3 Å resolution. Our atomic model reveals a typical major facilitator superfamily fold, with the substrate-binding site open to the extracellular side of the membrane. Despite the nearly identical conformation to its closest family member, rat GLUT5, our structure unveils distinct features of the substrate-binding cavity that may influence substrate specificity and binding mode. A homology model of the inward-open human GLUT7 indicates that similar to other members of the GLUT family, it may undergo a global rocker-switch-like reorientation of the transmembrane bundles to facilitate substrate translocation across the membrane. Our work enhances the current structural understanding of the GLUT family, and lays a foundation for rational design of regulators of GLUTs and other sugar transporters. |
リンク | Biochem Biophys Res Commun / PubMed:39163817 |
手法 | EM (単粒子) |
解像度 | 3.3 Å |
構造データ | EMDB-61099: Cryo-EM structure of the human glucose transporter, GLUT7 in outward-facing open conformation |
由来 |
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