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-Structure paper
タイトル | Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 21, Issue 12, Page 1075-1081, Year 2014 |
掲載日 | 2014年11月2日 |
![]() | Peng Ge / Zeynep A Oztug Durer / Dmitri Kudryashov / Z Hong Zhou / Emil Reisler / ![]() |
PubMed 要旨 | Essential cellular processes involving the actin cytoskeleton are regulated by auxiliary proteins that can sense the nucleotide state of actin. Here we report cryo-EM structures for ADP-bound and ADP- ...Essential cellular processes involving the actin cytoskeleton are regulated by auxiliary proteins that can sense the nucleotide state of actin. Here we report cryo-EM structures for ADP-bound and ADP-beryllium fluoride (ADP-BeFx, an ADP-Pi mimic)-bound actin filaments in complex with the β-propeller domain of yeast coronin 1 (crn1), at 8.6-Å resolution. Our structures reveal the main differences in the interaction of coronin with the two nucleotide states of F-actin. We derived pseudoatomic models by fitting the atomic structures of actin and coronin into the EM envelopes and confirmed the identified interfaces on actin by chemical cross-linking, fluorescence spectroscopy and actin mutagenesis. The models offer a structural explanation for the nucleotide-dependent effects of coronin on cofilin-assisted remodeling of F-actin. |
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手法 | EM (らせん対称) |
解像度 | 8.6 Å |
構造データ | ![]() EMDB-6100: ![]() EMDB-6101: |
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