EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 9056, Year 2024
掲載日	2024年10月20日
著者	Lianyun Lin / Changshi Wang / Wenlan Wang / Heng Jiang / Takashi Murayama / Takuya Kobayashi / Hadiatullah Hadiatullah / Yu Seby Chen / Shunfan Wu / Yiwen Wang / Henryk Korza / Yucheng Gu / Yan Zhang / Jiamu Du / Filip Van Petegem / Zhiguang Yuchi /
PubMed 要旨	The resistance of pests to common insecticides is a global issue that threatens food production worldwide. Diamide insecticides target insect ryanodine receptors (RyRs), causing uncontrolled calcium ...The resistance of pests to common insecticides is a global issue that threatens food production worldwide. Diamide insecticides target insect ryanodine receptors (RyRs), causing uncontrolled calcium release from the sarcoplasmic and endoplasmic reticulum. Despite their high potency and species selectivity, several resistance mutations have emerged. Using a chimeric RyR (chiRyR) approach and cryo-electron microscopy (cryo-EM), we investigate how insect RyRs engage two different diamide insecticides from separate families: flubendiamide, a phthalic acid derivative, and tetraniliprole, an anthranilic compound. Both compounds target the same site in the transmembrane region of the RyR, albeit with different poses, and promote channel opening through coupling with the pore-forming domain. To explore the resistance mechanisms, we also solve two cryo-EM structures of chiRyR carrying the two most common resistance mutations, I4790M and G4946E, both alone and in complex with the diamide insecticide chlorantraniliprole. The resistance mutations perturb the local structure, directly reducing the binding affinity and altering the binding pose. Our findings elucidate the mode of action of different diamide insecticides, reveal the molecular mechanism of resistance mutations, and provide important clues for the development of novel pesticides that can bypass the resistance mutations.
リンク	Nat Commun / PubMed:39428398 / PubMed Central
手法	EM (単粒子)
解像度	3.28 - 3.91 Å
構造データ	38398 8xji EMDB-38398, PDB-8xji: Structure of chimeric RyR complex with flubendiamide 手法: EM (単粒子) / 解像度: 3.91 Å 38417 8xkh EMDB-38417, PDB-8xkh: Structure of chimeric RyR Complex with tetraniliprole 手法: EM (単粒子) / 解像度: 3.87 Å 38447 8xlf EMDB-38447, PDB-8xlf: Structure of chimeric RyR 手法: EM (単粒子) / 解像度: 3.62 Å 38448 8xlh EMDB-38448, PDB-8xlh: Structure of chimeric RyR-I4657M/G4819E 手法: EM (単粒子) / 解像度: 3.62 Å EMDB-38551: The map of chimeric RyR transmembrane domain in complex with flubendiamide after TMD local refinement 手法: EM (単粒子) / 解像度: 3.82 Å EMDB-38553: The map of chimeric RyR transmembrane domain in complex with tetraniliprole with TMD local refinement 手法: EM (単粒子) / 解像度: 3.77 Å 38908 8y40 EMDB-38908, PDB-8y40: Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-60899: Structure of a chimeric RyR-I4657M/G4819E (local refinement of TMD) 手法: EM (単粒子) / 解像度: 3.28 Å EMDB-60900: Cryo-EM structure of ref-chiRyR (local refinement of TMD) 手法: EM (単粒子) / 解像度: 3.68 Å EMDB-60901: cryo-EM structure of chiRyR-I4657M/G4819E complex with CHL (local refinement of TMD) 手法: EM (単粒子) / 解像度: 3.37 Å
化合物	PDB-1lvx: Unknown entry ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-CFF: CAFFEINE / カフェイン / 薬剤YM PDB-1lv1: Crystal Structure Analysis of the non-active site mutant of tethered HIV-1 protease to 2.1A resolution ChemComp-F0U: 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide / クロラントラニリプロ-ル
由来	oryctolagus cuniculus (ウサギ) homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / Ryanodine receptor / Ion channel / tetraniliprole / chlorantraniliprole