EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural remodeling of bacteriophage T4 and host membranes during infection initiation.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 112, Issue 35, Page E4919-E4928, Year 2015
掲載日	2015年9月1日
著者	Bo Hu / William Margolin / Ian J Molineux / Jun Liu /
PubMed 要旨	The first stages of productive bacteriophage infections of bacterial host cells require efficient adsorption to the cell surface followed by ejection of phage DNA into the host cytoplasm. To achieve ...The first stages of productive bacteriophage infections of bacterial host cells require efficient adsorption to the cell surface followed by ejection of phage DNA into the host cytoplasm. To achieve this goal, a phage virion must undergo significant structural remodeling. For phage T4, the most obvious change is the contraction of its tail. Here, we use skinny E. coli minicells as a host, along with cryo-electron tomography and mutant phage virions, to visualize key structural intermediates during initiation of T4 infection. We show for the first time that most long tail fibers are folded back against the tail sheath until irreversible adsorption, a feature compatible with the virion randomly walking across the cell surface to find an optimal site for infection. Our data confirm that tail contraction is triggered by structural changes in the baseplate, as intermediates were found with remodeled baseplates and extended tails. After contraction, the tail tube penetrates the host cell periplasm, pausing while it degrades the peptidoglycan layer. Penetration into the host cytoplasm is accompanied by a dramatic local outward curvature of the cytoplasmic membrane as it fuses with the phage tail tip. The baseplate hub protein gp27 and/or the ejected tape measure protein gp29 likely form the transmembrane channel for viral DNA passage into the cell cytoplasm. Building on the wealth of prior biochemical and structural information, this work provides new molecular insights into the mechanistic pathway of T4 phage infection.
リンク	Proc Natl Acad Sci U S A / PubMed:26283379 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	31.0 - 40.0 Å
構造データ	EMDB-2774: Cryo electron tomography of infective bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 36.0 Å EMDB-6078: Cryo electron tomography of Wac-minus bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 38.0 Å EMDB-6079: Cryo electron tomography of fiberless bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 31.0 Å EMDB-6080: Cryo electron tomography of infective bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 40.0 Å EMDB-6081: Cryo electron tomography of gp9 minus bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 31.0 Å EMDB-6082: Cryo electron tomography of infective bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 31.0 Å EMDB-6083: Cryo electron tomography of infective bacteriophage T4 手法: EM (サブトモグラム平均) / 解像度: 33.0 Å