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-Structure paper
タイトル | Crystal structure of a nematode-infecting virus. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 111, Issue 35, Page 12781-12786, Year 2014 |
掲載日 | 2014年9月2日 |
著者 | Yusong R Guo / Corey F Hryc / Joanita Jakana / Hongbing Jiang / David Wang / Wah Chiu / Weiwei Zhong / Yizhi J Tao / |
PubMed 要旨 | Orsay, the first virus discovered to naturally infect Caenorhabditis elegans or any nematode, has a bipartite, positive-sense RNA genome. Sequence analyses show that Orsay is related to nodaviruses, ...Orsay, the first virus discovered to naturally infect Caenorhabditis elegans or any nematode, has a bipartite, positive-sense RNA genome. Sequence analyses show that Orsay is related to nodaviruses, but molecular characterizations of Orsay reveal several unique features, such as the expression of a capsid-δ fusion protein and the use of an ATG-independent mechanism for translation initiation. Here we report the crystal structure of an Orsay virus-like particle assembled from recombinant capsid protein (CP). Orsay capsid has a T = 3 icosahedral symmetry with 60 trimeric surface spikes. Each CP can be divided into three regions: an N-terminal arm that forms an extended protein interaction network at the capsid interior, an S domain with a jelly-roll, β-barrel fold forming the continuous capsid, and a P domain that forms surface spike projections. The structure of the Orsay S domain is best aligned to T = 3 plant RNA viruses but exhibits substantial differences compared with the insect-infecting alphanodaviruses, which also lack the P domain in their CPs. The Orsay P domain is remotely related to the P1 domain in calicivirus and hepatitis E virus, suggesting a possible evolutionary relationship. Removing the N-terminal arm produced a slightly expanded capsid with fewer nucleic acids packaged, suggesting that the arm is important for capsid stability and genome packaging. Because C. elegans-Orsay serves as a highly tractable model for studying viral pathogenesis, our results should provide a valuable structural framework for further studies of Orsay replication and infection. |
リンク | Proc Natl Acad Sci U S A / PubMed:25136116 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 3.25 - 6.9 Å |
構造データ | EMDB-5952: PDB-4nwv: PDB-4nww: |
化合物 | ChemComp-CA: |
由来 |
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キーワード | VIRUS / beta barrel |