EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Functional domains of the 50S subunit mature late in the assembly process.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 42, Issue 5, Page 3419-3435, Year 2014
掲載日	2013年12月13日
著者	Ahmad Jomaa / Nikhil Jain / Joseph H Davis / James R Williamson / Robert A Britton / Joaquin Ortega /
PubMed 要旨	Despite the identification of many factors that facilitate ribosome assembly, the molecular mechanisms by which they drive ribosome biogenesis are poorly understood. Here, we analyze the late stages ...Despite the identification of many factors that facilitate ribosome assembly, the molecular mechanisms by which they drive ribosome biogenesis are poorly understood. Here, we analyze the late stages of assembly of the 50S subunit using Bacillus subtilis cells depleted of RbgA, a highly conserved GTPase. We found that RbgA-depleted cells accumulate late assembly intermediates bearing sub-stoichiometric quantities of ribosomal proteins L16, L27, L28, L33a, L35 and L36. Using a novel pulse labeling/quantitative mass spectrometry technique, we show that this particle is physiologically relevant and is capable of maturing into a complete 50S particle. Cryo-electron microscopy and chemical probing revealed that the central protuberance, the GTPase associating region and tRNA-binding sites in this intermediate are unstructured. These findings demonstrate that key functional sites of the 50S subunit remain unstructured until late stages of maturation, preventing the incomplete subunit from prematurely engaging in translation. Finally, structural and biochemical analysis of a ribosome particle depleted of L16 indicate that L16 binding is necessary for the stimulation of RbgA GTPase activity and, in turn, release of this co-factor, and for conversion of the intermediate to a complete 50S subunit.
リンク	Nucleic Acids Res / PubMed:24335279 / PubMed Central
手法	EM (単粒子)
解像度	11.0 - 13.0 Å
構造データ	EMDB-5787: Cryo-electron microscopy of the 50S ribosomal subunit from Bacillus subtilis 手法: EM (単粒子) / 解像度: 11.0 Å EMDB-5788: Cryo-electron microscopy of the 50S ribosomal subunit from Bacillus subtilis without L16 ribosomal protein 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-5789: Cryo-electron microscopy of an immature 50S ribosomal subunit (45S particle) from Bacillus depleted of RbgA (YlqF), conformation 1 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-5790: Cryo-electron microscopy of an immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 2 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-5791: Cryo-electron microscopy of an immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 3 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-5792: Cryo-electron microscopy of an immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 4 手法: EM (単粒子) / 解像度: 13.0 Å
由来	Bacillus subtilis (枯草菌)