EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Influences on trimerization and aggregation of soluble, cleaved HIV-1 SOSIP envelope glycoprotein.
ジャーナル・号・ページ	J Virol, Vol. 87, Issue 17, Page 9873-9885, Year 2013
掲載日	2013年7月3日
著者	Per Johan Klasse / Rafael S Depetris / Robert Pejchal / Jean-Philippe Julien / Reza Khayat / Jeong Hyun Lee / Andre J Marozsan / Albert Cupo / Nicolette Cocco / Jacob Korzun / Anila Yasmeen / Andrew B Ward / Ian A Wilson / Rogier W Sanders / John P Moore /
PubMed 要旨	We describe methods to improve the properties of soluble, cleaved gp140 trimers of the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) for use in structural studies and as ...We describe methods to improve the properties of soluble, cleaved gp140 trimers of the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) for use in structural studies and as immunogens. In the absence of nonionic detergents, gp140 of the KNH1144 genotype, terminating at residue 681 in gp41 (SOSIP.681), has a tendency to form higher-order complexes or aggregates, which is particularly undesirable for structure-based research. We found that this aggregation in the absence of detergent does not involve the V1, V2, or V3 variable regions of gp120. Moreover, we observed that detergent forms micelles around the membrane-proximal external region (MPER) of the SOSIP.681 gp140 trimers, whereas deletion of most of the MPER residues by terminating the gp140 at residue 664 (SOSIP.664) prevented the aggregation that otherwise occurs in SOSIP.681 in the absence of detergent. Although the MPER can contribute to trimer formation, truncation of most of it only modestly reduced trimerization and lacked global adverse effects on antigenicity. Thus, the MPER deletion minimally influenced the kinetics of the binding of soluble CD4 and a CD4-binding site antibody to immobilized trimers, as detected by surface plasmon resonance. Furthermore, the MPER deletion did not alter the overall three-dimensional structure of the trimers, as viewed by negative-stain electron microscopy. Homogeneous and aggregate-free MPER-truncated SOSIP Env trimers are therefore useful for immunogenicity and structural studies.
リンク	J Virol / PubMed:23824824 / PubMed Central
手法	EM (単粒子)
解像度	14.0 - 22.0 Å
構造データ	EMDB-5700: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.681 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-5701: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.681.dV1V2V3 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-5702: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.681 in complex with Fab fragment of VRC PG04 monoclonal antibody 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-5703: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.681.dV1V2V3 in complex with Fab fragment of VRC PG04 monoclonal antibody 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-5704: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.681 in complex with soluble CD4 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-5705: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664 手法: EM (単粒子) / 解像度: 14.0 Å EMDB-5706: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664 in complex with Fab fragment of VRC PG04 monoclonal antibody 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-5707: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664.dV1V2V3 in complex with Fab fragment of VRC PG04 monoclonal antibody 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-5708: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664 in complex with soluble CD4 手法: EM (単粒子) / 解像度: 16.0 Å EMDB-5709: Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664.dV1V2V3 in complex with soluble CD4 手法: EM (単粒子) / 解像度: 19.0 Å
由来	Human immunodeficiency virus (ヒト免疫不全ウイルス) unidentified (未定義) Homo sapiens (ヒト)