EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryoelectron tomography reveals the sequential assembly of bacterial flagella in Borrelia burgdorferi.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 110, Issue 35, Page 14390-14395, Year 2013
掲載日	2013年8月27日
著者	Xiaowei Zhao / Kai Zhang / Tristan Boquoi / Bo Hu / M A Motaleb / Kelly A Miller / Milinda E James / Nyles W Charon / Michael D Manson / Steven J Norris / Chunhao Li / Jun Liu /
PubMed 要旨	Periplasmic flagella are essential for the distinctive morphology, motility, and infectious life cycle of the Lyme disease spirochete Borrelia burgdorferi. In this study, we genetically trapped ...Periplasmic flagella are essential for the distinctive morphology, motility, and infectious life cycle of the Lyme disease spirochete Borrelia burgdorferi. In this study, we genetically trapped intermediates in flagellar assembly and determined the 3D structures of the intermediates to 4-nm resolution by cryoelectron tomography. We provide structural evidence that secretion of rod substrates triggers remodeling of the central channel in the flagellar secretion apparatus from a closed to an open conformation. This open channel then serves as both a gateway and a template for flagellar rod assembly. The individual proteins assemble sequentially to form a modular rod. The hook cap initiates hook assembly on completion of the rod, and the filament cap facilitates filament assembly after formation of the mature hook. Cryoelectron tomography and mutational analysis thus combine synergistically to provide a unique structural blueprint of the assembly process of this intricate molecular machine in intact cells.
リンク	Proc Natl Acad Sci U S A / PubMed:23940315 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	35.0 - 39.0 Å
構造データ	EMDB-5627: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-5628: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FliE mutant 手法: EM (サブトモグラム平均) / 解像度: 39.0 Å EMDB-5629: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgB mutant 手法: EM (サブトモグラム平均) / 解像度: 39.0 Å EMDB-5630: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgC mutant 手法: EM (サブトモグラム平均) / 解像度: 38.0 Å EMDB-5631: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlhO mutant 手法: EM (サブトモグラム平均) / 解像度: 39.0 Å EMDB-5632: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgG mutant 手法: EM (サブトモグラム平均) / 解像度: 37.0 Å EMDB-5633: Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgE mutant 手法: EM (サブトモグラム平均) / 解像度: 38.0 Å
由来	Borrelia burgdorferi (バクテリア)