EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular structures of trimeric HIV-1 Env in complex with small antibody derivatives.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 110, Issue 2, Page 513-518, Year 2013
掲載日	2013年1月8日
著者	Joel R Meyerson / Erin E H Tran / Oleg Kuybeda / Weizao Chen / Dimiter S Dimitrov / Andrea Gorlani / Theo Verrips / Jeffrey D Lifson / Sriram Subramaniam /
PubMed 要旨	The extensive carbohydrate coat, the variability of protein structural features on HIV-1 envelope glycoproteins (Env), and the steric constraints of the virus-cell interface during infection, present ...The extensive carbohydrate coat, the variability of protein structural features on HIV-1 envelope glycoproteins (Env), and the steric constraints of the virus-cell interface during infection, present challenges to the elicitation of effective full-length (~150 kDa), neutralizing antibodies against HIV. These hurdles have motivated the engineering of smaller antibody derivatives that can bind Env and neutralize the virus. To further understand the mechanisms by which these proteins neutralize HIV-1, we carried out cryoelectron tomography of native HIV-1 BaL virions complexed separately to two small (~15 kDa) HIV-neutralizing proteins: A12, which binds the CD4-binding site on Env, and m36, whose binding to Env is enhanced by CD4 binding. We show that despite their small size, the presence of these proteins and their effects on the quaternary conformation of trimeric Env can be visualized in molecular structures derived by cryoelectron tomography combined with subvolume averaging. Binding of Env to A12 results in a conformational change that is comparable to changes observed upon its binding to the CD4-binding site antibody, b12. In contrast, binding of Env to m36 results in an "open" quaternary conformation similar to that seen with binding of soluble CD4 or the CD4i antibody, 17b. Because these small neutralizing proteins are less sterically hindered than full-length antibodies at zones of virus-cell contact, the finding that their binding has the same structural consequences as that of other broadly neutralizing antibodies highlights their potential for use in therapeutic applications.
リンク	Proc Natl Acad Sci U S A / PubMed:23267106 / PubMed Central
手法	EM (サブトモグラム平均)
構造データ	EMDB-5544: Molecular structure of the native HIV-1 Env trimer bound to VHH A12: Spike region 手法: EM (サブトモグラム平均) EMDB-5551: Molecular structure of the native HIV-1 Env trimer bound to VHH A12: Membrane region 手法: EM (サブトモグラム平均) EMDB-5552: Molecular structure of the native HIV-1 Env trimer bound to m36: Spike region 手法: EM (サブトモグラム平均) EMDB-5553: Molecular structure of the native HIV-1 Env trimer bound to m36: Membrane region 手法: EM (サブトモグラム平均) EMDB-5554: Molecular structure of the native HIV-1 Env trimer bound to m36 and sCD4: Spike region 手法: EM (サブトモグラム平均) EMDB-5555: Molecular structure of the native HIV-1 Env trimer bound to m36 and sCD4: Membrane region 手法: EM (サブトモグラム平均)
由来	Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)