EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Tangled up in knots: structures of inactivated forms of E. coli class Ia ribonucleotide reductase.
ジャーナル・号・ページ	Structure, Vol. 20, Issue 8, Page 1374-1383, Year 2012
掲載日	2012年8月8日
著者	Christina M Zimanyi / Nozomi Ando / Edward J Brignole / Francisco J Asturias / Joanne Stubbe / Catherine L Drennan /
PubMed 要旨	Ribonucleotide reductases (RNRs) provide the precursors for DNA biosynthesis and repair and are successful targets for anticancer drugs such as clofarabine and gemcitabine. Recently, we reported that ...Ribonucleotide reductases (RNRs) provide the precursors for DNA biosynthesis and repair and are successful targets for anticancer drugs such as clofarabine and gemcitabine. Recently, we reported that dATP inhibits E. coli class Ia RNR by driving formation of RNR subunits into α4β4 rings. Here, we present the first X-ray structure of a gemcitabine-inhibited E. coli RNR and show that the previously described α4β4 rings can interlock to form an unprecedented (α4β4)2 megacomplex. This complex is also seen in a higher-resolution dATP-inhibited RNR structure presented here, which employs a distinct crystal lattice from that observed in the gemcitabine-inhibited case. With few reported examples of protein catenanes, we use data from small-angle X-ray scattering and electron microscopy to both understand the solution conditions that contribute to concatenation in RNRs as well as present a mechanism for the formation of these unusual structures.
リンク	Structure / PubMed:22727814 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.95 - 33.4 Å
構造データ	EMDB-5430: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 closed ring conformation 手法: EM (単粒子) / 解像度: 23.4 Å EMDB-5431: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 1 手法: EM (単粒子) / 解像度: 32.0 Å EMDB-5432: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 2 手法: EM (単粒子) / 解像度: 32.1 Å EMDB-5433: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 3 手法: EM (単粒子) / 解像度: 31.5 Å EMDB-5434: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 4 手法: EM (単粒子) / 解像度: 29.8 Å EMDB-5435: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 5 手法: EM (単粒子) / 解像度: 33.4 Å EMDB-5436: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 6 手法: EM (単粒子) / 解像度: 29.3 Å EMDB-5437: dATP-inhibited class Ia ribonucleotide reductase from E. coli: alpha4beta4 open conformation 7 手法: EM (単粒子) / 解像度: 33.1 Å PDB-4erm: Crystal structure of the dATP inhibited E. coli class Ia ribonucleotide reductase complex at 4 Angstroms resolution 手法: X-RAY DIFFRACTION / 解像度: 3.95 Å PDB-4erp: Crystal structure of a gemcitabine-diphosphate inhibited E. coli class Ia ribonucleotide reductase complex 手法: X-RAY DIFFRACTION / 解像度: 4.45 Å
化合物	ChemComp-DTP: 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE / dATP / デオキシアデノシン三リン酸 ChemComp-DAT: 2'-DEOXYADENOSINE-5'-DIPHOSPHATE / dADP / デオキシアデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-FEO: MU-OXO-DIIRON ChemComp-HOH: WATER / 水 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM / アデノシン三リン酸
由来	Escherichia coli (大腸菌) escherichia coli k-12 (大腸菌)
キーワード	OXIDOREDUCTASE (酸化還元酵素) / protein-protein complex / alpha/beta barrel / atp cone / di-iron center / RNR alpha / RNR beta / thioredoxin (チオレドキシン) / Ribonucleotide reduction / Cytosol (細胞質基質) / diiron center