EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for Rep-mediated adeno-associated virus packaging.
ジャーナル・号・ページ	Cell Rep, Vol. 45, Issue 3, Page 117044, Year 2026
掲載日	2026年3月5日
著者	Victoria Xu / Aiysha McInnes / Matthew Wake / Marta Acebrón-García-de-Eulate / Joseph D Barritt / Doryen Bubeck / Sarah L Rouse /
PubMed 要旨	Adeno-associated viruses (AAVs) are parvoviruses utilized as gene therapy vectors. However, the AAV packaging mechanism is unresolved at the molecular level, creating a bottleneck for vector ...Adeno-associated viruses (AAVs) are parvoviruses utilized as gene therapy vectors. However, the AAV packaging mechanism is unresolved at the molecular level, creating a bottleneck for vector manufacturing, safety, and efficacy. Here, cryo-EM structures of the Rep helicase packaging motor in complex with the packaging marker DNA (ITR) and the Rep-AAV8 capsid complex are presented. Rep-ITR complexes reveal dynamic oligomeric states on the DNA, elucidating the strand separation mechanism coupled to its ATPase cycle. We observe Rep preferentially bound to empty capsids, with a binding interface likely conserved across the virus family. This complex also unveils a cryptic capsid ATP-binding site which, alongside Rep binding, triggers structural rearrangements priming the capsid for packaging. Collectively, these findings advance the understanding of Rep-mediated packaging, with significant implications for parvovirus virology and viral vector design.
リンク	Cell Rep / PubMed:41790558
手法	EM (単粒子)
解像度	1.88 - 3.08 Å
構造データ	54050 9rm5 EMDB-54050, PDB-9rm5: Hexameric AAV2 Rep40-ssDNA (ITR) complex in presence of ATPyS 手法: EM (単粒子) / 解像度: 2.44 Å EMDB-54181: Consensus map of heptameric Rep40-dsDNA (ITR) in presence of ATPyS 手法: EM (単粒子) / 解像度: 2.75 Å EMDB-54182: Focused map of 3 subunits of Rep40 +dsDNA (ITR) in complex with ATPgS 手法: EM (単粒子) / 解像度: 2.63 Å EMDB-54183: Focused map of 4 subunits of heptameric Rep40-dsDNA (ITR) in complex with ATPgS 手法: EM (単粒子) / 解像度: 2.96 Å 54184 9rqt EMDB-54184, PDB-9rqt: Heptameric AAV2 Rep40-dsDNA (ITR) complex in presence of ATPgS 手法: EM (単粒子) / 解像度: 2.88 Å 54195 9rrs EMDB-54195, PDB-9rrs: AAV8 capsid in complex with Rep40 and ADP 手法: EM (単粒子) / 解像度: 1.88 Å 54328 9rwg EMDB-54328, PDB-9rwg: Pentameric AAV2 Rep40 in complex with AAV8 手法: EM (単粒子) / 解像度: 2.19 Å EMDB-54403: Consensus map of Hexameric AAV2 Rep40-dsDNA (ITR) duplex complex in presence of ATPyS 手法: EM (単粒子) / 解像度: 3.07 Å 54416 9s0n EMDB-54416, PDB-9s0n: Hexameric AAV2 Rep40-dsDNA (ITR) melting complex in presence of ATPyS 手法: EM (単粒子) / 解像度: 3.08 Å 54429 9s10 EMDB-54429, PDB-9s10: Hexameric AAV2 Rep40-dsDNA (ITR) duplex complex in presence of ATPyS 手法: EM (単粒子) / 解像度: 3.07 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-HOH: WATER
由来	adeno-associated virus 2 (アデノ随伴ウイルス) dna molecule (その他) adeno-associated virus 8 (アデノ随伴ウイルス)
キーワード	DNA BINDING PROTEIN / Helicase / Adeno-associated Virus / AAA+ ATPase / VIRUS / AAV