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-Structure paper
タイトル | Structure of the Fab-labeled "breathing" state of native poliovirus. |
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ジャーナル・号・ページ | J Virol, Vol. 86, Issue 10, Page 5959-5962, Year 2012 |
掲載日 | 2012年3月7日 |
![]() | Jun Lin / Lily Y Lee / Merja Roivainen / David J Filman / James M Hogle / David M Belnap / ![]() |
PubMed 要旨 | At 37°C, the structure of poliovirus is dynamic, and internal polypeptides VP4 and N terminus of VP1 (residues 1 to 53) externalize reversibly. An Fab fragment of a monospecific antibody, which ...At 37°C, the structure of poliovirus is dynamic, and internal polypeptides VP4 and N terminus of VP1 (residues 1 to 53) externalize reversibly. An Fab fragment of a monospecific antibody, which binds to residues 39 to 55 of VP1, was utilized to locate the N termini of VP1 in native (160S) particles in this "breathing" state. Fab and virus were mixed and imaged via cryogenic electron microscopy. The resulting reconstruction showed the capsid expands similarly to the irreversibly altered cell entry intermediate (135S) particle, but the N terminus of VP1 is located near the 2-fold axes, instead of the "propeller tip" as in 135S particles. |
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手法 | EM (単粒子) |
解像度 | 25.0 Å |
構造データ | ![]() EMDB-5387: |