ムービー
コントローラー
構造ビューア
万見文献について
+検索条件
-Structure paper
| タイトル | Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles. |
|---|---|
| ジャーナル・号・ページ | Commun Biol, Vol. 9, Issue 1, Page 226, Year 2026 |
| 掲載日 | 2026年1月13日 |
 著者 | Neža Koritnik / Andreja Kežar / Luka Kavčič / Magda Tušek Žnidarič / Adrijana Leonardi / Swarnalok De / Maija Pollari / Kristiina Mäkinen / Marjetka Podobnik /   |
| PubMed 要旨 | Potyviruses are the largest group of plant positive-sense single-stranded RNA viruses and represent a major economic burden worldwide. Their coat protein (CP) forms a filamentous, flexible capsid ...Potyviruses are the largest group of plant positive-sense single-stranded RNA viruses and represent a major economic burden worldwide. Their coat protein (CP) forms a filamentous, flexible capsid around the genomic RNA. However, information is still lacking on the mechanisms of virion assembly, disassembly and stability, which is central to understanding virus biology and control. Here, we investigate the role of CP in these processes using structural, biochemical and biophysical studies of five potyviral CPs from three phylogenetic clades combined with bioinformatics and in planta experiments. Our results suggest that, while potyviruses have a conserved virion structure, the amino acids forming the CP-CP and CP-RNA interactions leading to this structure are species-specific. We show that the species-specific CP sequence also determines the architecture of RNA-free virus-like particles (VLPs) and the degree of their structural polymorphism. We identify the residues that determine this specificity at distinct S1-S4 interaction sites. In contrast, a highly conserved charged amino acid triad at the CP-CP interface is essential for the stability of virions and RNA-free VLPs. These results contribute to understanding the molecular mechanism of potyviral virion assembly and highlight the significance of the amino acid sequence of selected CPs in potential biotechnological or biomedical applications. |
 リンク | Commun Biol / PubMed:41530503 / PubMed Central |
| 手法 | EM (らせん対称) |
| 解像度 | 2.33 - 6.84 Å |
| 構造データ |  EMDB-53632: ssRNA-containing helical virus-like particle composed of PVA coat protein with 5 mutations (H89Q, E90S, S144E, K180E, and T210R)  EMDB-53633: RNA-free helical (h8.7) virus-like particle composed of PVA coat protein with 5 mutations (H89Q, E90S, S144E, K180E, and T210R)  EMDB-53634: RNA-free helical (h9.7) virus-like particle composed of PVA coat protein with 5 mutations (H89Q, E90S, S144E, K180E, and T210R)  EMDB-53635: RNA-free stacked-ring (r10) virus-like particle composed of PVA coat protein with 5 mutations (H89Q, E90S, S144E, K180E, and T210R)  EMDB-53636: RNA-free helical (h8.5) virus-like particle composed of PVA coat protein with R163A mutation  EMDB-53638: ssRNA-containing helical virus-like particle composed of PVY coat protein with Q87H mutation  EMDB-53639: RNA-free helical (h8.5) virus-like particle composed of PVY coat protein with Q87H mutation  EMDB-53640: RNA-free stacked-ring (r8) virus-like particle composed of PVY coat protein with Q87H mutation  EMDB-53642: ssRNA-containing helical virus-like particle composed of PVY coat protein with R208T mutation  EMDB-53643: RNA-free helical (h7.5) virus-like particle composed of PVY coat protein with R208T mutation  EMDB-53644: RNA-free helical (h8.5) virus-like particle composed of PVY coat protein with R208T mutation  EMDB-53645: RNA-free stacked-ring (r8) virus-like particle composed of PVY coat protein with R208T mutation  EMDB-53646: ssRNA-containing helical virus-like particle composed of PVY coat protein with Q87H and R208T mutations  EMDB-53647: RNA-free helical (h8.5) virus-like particle composed of PVY coat protein with Q87H and R208T mutations  EMDB-53650: ssRNA-containing helical virus-like particle composed of PVY coat protein with A161R mutation  EMDB-53651: RNA-free helical (h8.5) virus-like particle composed of PVA coat protein (in vitro assembly) EMDB-53790, PDB-9r7r: EMDB-53791, PDB-9r7s: EMDB-53792, PDB-9r7t: EMDB-53793, PDB-9r7u: EMDB-53794, PDB-9r7v: EMDB-53796, PDB-9r7x: EMDB-53799, PDB-9r7y: EMDB-53800, PDB-9r7z: EMDB-53801, PDB-9r80: EMDB-53802, PDB-9r81: EMDB-53862, PDB-9r9w: EMDB-53863, PDB-9r9x: EMDB-53864, PDB-9r9y: EMDB-53865, PDB-9r9z: EMDB-53866, PDB-9ra0: EMDB-53867, PDB-9ra1: EMDB-53868, PDB-9ra2: |
| 由来 |
|
 キーワード | VIRUS / potato virus A / potyvirus / coat protein / RNA / helical / VIRUS LIKE PARTICLE / virus-like particle / tobacco etch virus / pepper mottle virus / Johnsongrass mosaic virus |
potato virus a (ウイルス)