EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic insights into Bcs1-mediated mitochondrial membrane translocation of the folded Rieske protein.
ジャーナル・号・ページ	EMBO J, Vol. 44, Issue 13, Page 3720-3741, Year 2025
掲載日	2025年5月23日
著者	Cristian Rosales-Hernandez / Matthias Thoms / Otto Berninghausen / Thomas Becker / Roland Beckmann /
PubMed 要旨	A functional mitochondrial respiratory chain requires coordinated and tightly regulated assembly of mitochondrial- and nuclear-encoded subunits. For bc1 complex (complex III) assembly, the iron- ...A functional mitochondrial respiratory chain requires coordinated and tightly regulated assembly of mitochondrial- and nuclear-encoded subunits. For bc1 complex (complex III) assembly, the iron-sulfur protein Rip1 must first be imported into the mitochondrial matrix to fold and acquire its 2Fe-2S cluster, then translocated and inserted into the inner mitochondrial membrane (IM). This translocation of folded Rip1 is accomplished by Bcs1, an unusual heptameric AAA ATPase that couples ATP hydrolysis to translocation. However, the molecular and mechanistic details of Bcs1-mediated Rip1 translocation have remained elusive. Here, we provide structural and biochemical evidence on how Bcs1 alternates between conformational states to translocate Rip1 across the IM. Using cryo-electron microscopy (cryo-EM), we identified substrate-bound pre-translocation and pre-release states, revealing how electrostatic interactions promote Rip1 binding to Bcs1. An ATP-induced conformational switch of the Bcs1 heptamer facilitates Rip1 translocation between two distinct aqueous vestibules-one exposed to the matrix, the other to the intermembrane space-in an airlock-like mechanism. This would minimize disruption of the IM permeability barrier, which could otherwise lead to proton leakage and compromised mitochondrial energy conversion.
リンク	EMBO J / PubMed:40410623 / PubMed Central
手法	EM (単粒子)
解像度	2.58 - 3.56 Å
構造データ	51537 9gs2 EMDB-51537, PDB-9gs2: Structure of the Rieske bound Apo1 state of the heptameric Bcs1 AAA-ATPase 手法: EM (単粒子) / 解像度: 3.46 Å 51552 9gsn EMDB-51552, PDB-9gsn: Structure of the ATPgS-S1 state of the heptameric Bcs1 AAA-ATPase 手法: EM (単粒子) / 解像度: 2.58 Å EMDB-51561: Heptameric AAA-ATPase Bcs1 in complex with bc1 mitochondrial subunit Rieske 手法: EM (単粒子) / 解像度: 3.56 Å EMDB-51562: Heptameric AAA-ATPase Bcs1 in the ATPgS2 state bound to Rieske-ISP 手法: EM (単粒子) / 解像度: 3.07 Å 51605 9gu9 EMDB-51605, PDB-9gu9: Structure of the ATPgS-S2 state of the heptameric Bcs1 AAA-ATPase 手法: EM (単粒子) / 解像度: 2.74 Å EMDB-53185: ATPgS-S1 (C1) state of the heptameric Bcs1 AAA-ATPase 手法: EM (単粒子) / 解像度: 3.05 Å EMDB-53189: ATPgS-S2 (C1) state of the heptameric Bcs1 AAA-ATPase 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体*YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	TRANSLOCASE / Heptameric complex Iron-sulfur cluster substrate / Heptameric complex / mitochondrial