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-Structure paper
| タイトル | Seeing the portal in herpes simplex virus type 1 B capsids. |
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| ジャーナル・号・ページ | J Virol, Vol. 85, Issue 4, Page 1871-1874, Year 2011 |
| 掲載日 | 2010年11月24日 |
著者 | R H Rochat / X Liu / K Murata / K Nagayama / F J Rixon / W Chiu / ![]() |
| PubMed 要旨 | Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron ...Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal. |
リンク | J Virol / PubMed:21106752 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 24.0 Å |
| 構造データ | ![]() EMDB-5255: ![]() EMDB-5259: ![]() EMDB-5260: ![]() EMDB-5261: |
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