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-Structure paper
| タイトル | Discrete structure of an RNA folding intermediate revealed by cryo-electron microscopy. |
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| ジャーナル・号・ページ | J Am Chem Soc, Vol. 132, Issue 46, Page 16352-16353, Year 2010 |
| 掲載日 | 2010年11月24日 |
著者 | Nathan J Baird / Steven J Ludtke / Htet Khant / Wah Chiu / Tao Pan / Tobin R Sosnick / ![]() |
| PubMed 要旨 | RNA folding occurs via a series of transitions between metastable intermediate states. It is unknown whether folding intermediates are discrete structures folding along defined pathways or ...RNA folding occurs via a series of transitions between metastable intermediate states. It is unknown whether folding intermediates are discrete structures folding along defined pathways or heterogeneous ensembles folding along broad landscapes. We use cryo-electron microscopy and single-particle image reconstruction to determine the structure of the major folding intermediate of the specificity domain of a ribonuclease P ribozyme. Our results support the existence of a discrete conformation for this folding intermediate. |
リンク | J Am Chem Soc / PubMed:21038867 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 15.0 - 15.2 Å |
| 構造データ | ![]() EMDB-5242: ![]() EMDB-5243: |
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