EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of iron piracy by human gut .
ジャーナル・号・ページ	bioRxiv, Year 2025
掲載日	2025年8月24日
著者	Augustinas Silale / Yung Li Soo / Hannah Mark / Rachel N Motz / Arnaud Baslé / Elizabeth M Nolan / Bert van den Berg /
PubMed 要旨	Iron is an essential element that can be growth-limiting in microbial communities, particularly those present within host organisms. To acquire iron, many bacteria secrete siderophores, secondary ...Iron is an essential element that can be growth-limiting in microbial communities, particularly those present within host organisms. To acquire iron, many bacteria secrete siderophores, secondary metabolites that chelate ferric iron. These iron chelates can be transported back into the cell via TonB-dependent transporters in the outer membrane, followed by intracellular liberation of the iron. Pathogenic and produce siderophores during gut infection. In response to iron starvation, the human gut symbiont upregulates an iron piracy system, XusABC, which steals iron-bound siderophores from the invading pathogens. Here, we investigated the molecular details of xenosiderophore uptake across the outer membrane by the XusAB complex. Our crystal and cryogenic electron microscopy structures explain how the XusB lipoprotein recognises iron-bound xenosiderophores and passes them on to the XusA TonB-dependent transporter. Moreover, we show that Xus homologues can transport a variety of siderophores with different iron-chelating functional groups.
リンク	bioRxiv / PubMed:40894706 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.5 - 3.32 Å
構造データ	51210 9gbc EMDB-51210, PDB-9gbc: Bacteroides thetaiotaomicron siderophore transporter XusA in complex with surface-exposed lipoprotein XusB 手法: EM (単粒子) / 解像度: 2.7 Å PDB-9gar: Siderophore-binding lipoprotein XusB from Barnesiella viscericola 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å PDB-9gcy: Xenosiderophore-binding lipoprotein XusB from Bacteroides thetaiotaomicron 手法: X-RAY DIFFRACTION / 解像度: 1.56 Å PDB-9gcz: XusB lipoprotein bound to ferric enterobactin 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å PDB-9hq1: XusB lipoprotein bound to ferric salmochelin 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-9hqe: Bacteroides fragilis xenosiderophore-binding lipoprotein XusB 手法: X-RAY DIFFRACTION / 解像度: 1.77 Å PDB-9hqk: Bacteroides fragilis lipoprotein XusB bound to ferrichrome 手法: X-RAY DIFFRACTION / 解像度: 3.32 Å
化合物	ChemComp-SO4: SULFATE ION ChemComp-ACT: ACETATE ION ChemComp-HOH: WATER ChemComp-CL: Unknown entry ChemComp-MES: 2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH緩衝剤YM ChemComp-CA: Unknown entry ChemComp-CIT: CITRIC ACID ChemComp-FE: Unknown entry ChemComp-EB4: N,N',N''-[(3S,7S,11S)-2,6,10-trioxo-1,5,9-trioxacyclododecane-3,7,11-triyl]tris(2,3-dihydroxybenzamide) ChemComp-DMS: DIMETHYL SULFOXIDE / DMSO, 沈殿剤YM ChemComp-NA: Unknown entry PDB-1iwm: Crystal Structure of the Outer Membrane Lipoprotein Receptor, LolB ChemComp-PGE: TRIETHYLENE GLYCOL ChemComp-PEG: DI(HYDROXYETHYL)ETHER ChemComp-FCE: FERRICHROME
由来	bacteroides thetaiotaomicron vpi-5482 (バクテリア) barnesiella viscericola dsm 18177 (バクテリア) bacteroides fragilis nctc 9343 (バクテリア)
キーワード	METAL TRANSPORT / Outer membrane / lipoprotein / siderophore / iron piracy / membrane protein complex / siderophore transport / METAL BINDING PROTEIN / xenosiderophore / Bacteroides / iron