EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 4720, Year 2025
掲載日	2025年5月21日
著者	Paulina Fischer / Matthias Thoms / Benjamin Lau / Timo Denk / Maria Kuvshinova / Otto Berninghausen / Dirk Flemming / Ed Hurt / Roland Beckmann /
PubMed 要旨	Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, ...Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, composed of pre-18S rRNA, ribosomal S-proteins, U3 snoRNA, and ~70 assembly factors. However, how numerous snoRNAs control pre-rRNA modification and folding during early maturation events remains unclear. We identify snR30 (human U17), the only essential H/ACA snoRNA in yeast, which binds with Cbf5-Gar1-Nop10-Nhp2 to a pre-18S rRNA subdomain containing platform helices and ES6 of the 40S central domain. Integration into the 90S is blocked by RNA hybridization with snR30. The snoRNP complex coordinates the recruitment of early assembly factors Krr1-Utp23-Kri1 and ribosomal proteins uS11-uS15, enabling isolated subdomain assembly. Krr1-dependent release of snR30 culminates in integration of the platform into the 90S. Our study reveals the essential role of snR30 in chaperoning central domain formation as a discrete assembly unit externalized from the pre-ribosomal core.
リンク	Nat Commun / PubMed:40399280 / PubMed Central
手法	EM (単粒子)
解像度	2.83 - 3.93 Å
構造データ	EMDB-50647: Stalled 90S - Utp23-Krr1-deltaC3 - Consensus refinement 手法: EM (単粒子) / 解像度: 3.05 Å EMDB-50648: Stalled 90S - Utp23-Krr1-deltaC3 - Head-Kre33 module - local refinement 手法: EM (単粒子) / 解像度: 3.51 Å EMDB-50649: Stalled 90S - Utp23-Krr1-deltaC3 - Krr1 - local refinement 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-50650: Stalled 90S - Utp23-Krr1-deltaC3 - Nop14 module - local refinement 手法: EM (単粒子) / 解像度: 3.25 Å EMDB-50651: Stalled 90S - Utp23-Krr1-deltaC3 - Utp10 - local refinement 手法: EM (単粒子) / 解像度: 3.59 Å EMDB-50652: Stalled 90S - Utp23-Krr1-deltaC3 - Utp12 - local refinement 手法: EM (単粒子) / 解像度: 3.38 Å EMDB-50653: Stalled 90S - Utp23-Krr1-deltaC3 - Utp20 - local refinement 手法: EM (単粒子) / 解像度: 3.23 Å EMDB-50654: Stalled 90S - Utp23-Krr1-deltaC3 - UTP-A - local refinement 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-50958: snR30 snoRNP - State 1 - Utp23-Krr1-deltaC3 - 3' HACA - local refinement 手法: EM (単粒子) / 解像度: 2.83 Å EMDB-50959: snR30 snoRNP - State 1 - Utp23-Krr1-deltaC3 - 5' HACA - local refinement 手法: EM (単粒子) / 解像度: 2.84 Å EMDB-50960: snR30 snoRNP - State 1 - Utp23-Krr1-deltaC3 - Platform module - local refinement 手法: EM (単粒子) / 解像度: 2.94 Å EMDB-50961: snR30 snoRNP - State 1 - Utp23-Krr1-deltaC3 - Consensus refinement 手法: EM (単粒子) / 解像度: 2.89 Å 50964 9g25 EMDB-50964, PDB-9g25: snR30 snoRNP - State 1 - Utp23-Krr1-deltaC3 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-50967: snR30 snoRNP - Class 1 - Utp23-Krr1-wt 手法: EM (単粒子) / 解像度: 3.92 Å 50968 9g28 EMDB-50968, PDB-9g28: snR30 snoRNP - State 2 - Utp23-Krr1-deltaC3 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-50969: snR30 snoRNP - Class 2 - Utp23-Krr1-wt 手法: EM (単粒子) / 解像度: 3.93 Å 50991 9g33 EMDB-50991, PDB-9g33: Stalled 90S - Utp23-Krr1-deltaC3 手法: EM (単粒子) / 解像度: 3.05 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子*YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	RIBOSOME / 90S / pre-ribosome / snoRNA / snR30 / snoRNP / ribosome biogenesis / H/ACA / 18S rRNA