EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir.
ジャーナル・号・ページ	Sci Adv, Vol. 11, Issue 45, Page eadz1989, Year 2025
掲載日	2025年11月7日
著者	Andrey G Baranovskiy / Qixiang He / Yoshiaki Suwa / Lucia M Morstadt / Nigar D Babayeva / Ci Ji Lim / Tahir H Tahirov /
PubMed 要旨	Widespread herpesvirus infections are associated with various diseases. DNA replication of human herpes simplex virus type 1 (HSV-1) requires a helicase-primase (HP) complex of three core proteins: ...Widespread herpesvirus infections are associated with various diseases. DNA replication of human herpes simplex virus type 1 (HSV-1) requires a helicase-primase (HP) complex of three core proteins: UL5, UL52, and UL8. This complex unwinds viral DNA and synthesizes primers for DNA replication, making it an attractive antiviral target. Although HP inhibitors pritelivir and amenamevir were identified through screening, their binding mechanisms remain unclear. Here, we report cryo-electron microscopy structures of HSV-1 HP bound to a forked DNA template alone and in complex with pritelivir or amenamevir. The structures reveal a bilobed architecture highlighting HP coordinated action at the replication fork and providing a structural basis for HP inhibition by illustrating precisely how pritelivir and amenamevir block helicase activity. Data lay a solid foundation for the development of improved antiviral therapies.
リンク	Sci Adv / PubMed:41202142 / PubMed Central
手法	EM (単粒子)
解像度	2.86 - 3.43 Å
構造データ	EMDB-49560: Consensus map of HSV-1 helicase-primase in complex with a forked DNA and amenamevir 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-49561: Focused map of UL5-UL52 (C-terminal and N-terminal) of HSV-1 helicase-primase in complex with a forked DNA and amenamevir 手法: EM (単粒子) / 解像度: 2.86 Å EMDB-49562: Focused map of UL8-UL52(410-893) of HSV-1 helicase-primase in complex with a forked DNA and amenamevir 手法: EM (単粒子) / 解像度: 2.96 Å 49563 9nn2 EMDB-49563, PDB-9nn2: Composite structure of HSV-1 helicase-primase in complex with a forked DNA and amenamevir 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-49582: Consensus map of HSV1 helicase-primase in complex with a forked DNA 手法: EM (単粒子) / 解像度: 3.43 Å EMDB-49583: Focused map of UL5-UL52 (C-terminal and N-terminal) of HSV-1 helicase-primase in complex with a forked DNA 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-49584: Focused map of UL8-UL52 (410-893) of HSV-1 helicase-primase in complex with a forked DNA 手法: EM (単粒子) / 解像度: 3.18 Å 49585 9nnp EMDB-49585, PDB-9nnp: Composite structure of HSV-1 helicase-primase in complex with a forked DNA 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-49586: Consensus map of HSV-1 helicase-primase in complex with a forked DNA and pritelivir 手法: EM (単粒子) / 解像度: 3.42 Å EMDB-49587: Focused map of UL52-UL5 (C-terminal and N-terminal) of HSV-1 helicase-primase in complex with a forked DNA and pritelivir 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-49588: Focused map of UL8-UL52 (417-890) of HSV-1 helicase-primase in complex with a forked DNA and pritelivir 手法: EM (単粒子) / 解像度: 3.2 Å 49669 9nqp EMDB-49669, PDB-9nqp: Composite structure of HSV1 helicase-primase in complex with a forked DNA and pritelivir 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	PDB-1bxd: NMR STRUCTURE OF THE HISTIDINE KINASE DOMAIN OF THE E. COLI OSMOSENSOR ENVZ ChemComp-ZN: Unknown entry ChemComp-HOH: WATER PDB-1bxb: XYLOSE ISOMERASE FROM THERMUS THERMOPHILUS
由来	human alphaherpesvirus 1 strain 17 (ヘルペスウイルス) homo sapiens (ヒト)
キーワード	Transferase/Hydrolase / DNA replication / HSV-1 helicase-primase / VIRAL PROTEIN / Transferase-Hydrolase complex / Transferase/Hydrolase/DNA / Transferase-Hydrolase-DNA complex / VIRAL PROTEIN/DNA / VIRAL PROTEIN-DNA complex