EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of a pentameric ligand-gated ion channel in liposomes.
ジャーナル・号・ページ	Elife, Vol. 14, Year 2025
掲載日	2025年7月16日
著者	Vikram Dalal / Brandon K Tan / Hanrui Xu / Wayland W L Cheng /
PubMed 要旨	Detergents and lipid nanodiscs affect the cryo-EM structures of pentameric ligand-gated ion channels (pLGICs) including ELIC. To determine the structure of a pLGIC in a membrane environment that ...Detergents and lipid nanodiscs affect the cryo-EM structures of pentameric ligand-gated ion channels (pLGICs) including ELIC. To determine the structure of a pLGIC in a membrane environment that supports ion channel function, we performed single particle cryo-EM of ELIC in liposomes. ELIC activation and desensitization were confirmed in liposomes with a stopped-flow thallium flux assay. Using WT ELIC and a non-desensitizing mutant (ELIC5), we captured resting, activated, and desensitized structures at high resolution. In the desensitized structure, the ion conduction pore has a constriction at the 9' leucine of the pore-lining M2 helix, indicating that 9' is the desensitization gate in ELIC. The agonist-bound structures of ELIC in liposomes are distinct from those in nanodiscs. In general, the transmembrane domain is more loosely packed in liposomes compared to nanodiscs. It has been suggested that large nanodiscs are superior for supporting membrane protein function. However, ELIC localizes to the rim of large circularized nanodiscs, and structures of ELIC in large nanodiscs deviate from the liposome structures more than those in small nanodiscs. Using liposomes for cryo-EM structure determination of a pLGIC increases our confidence that the structures are snapshots of functional states.
リンク	Elife / PubMed:40668221 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.3 Å
構造データ	49382 9ngc EMDB-49382, PDB-9ngc: ELIC5 with propylamine facing ECD outwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 2.8 Å 49383 9ngf EMDB-49383, PDB-9ngf: ELIC facing ECD outwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 3.4 Å 49384 9ngg EMDB-49384, PDB-9ngg: ELIC facing ECD inwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 3.6 Å 49385 9ngi EMDB-49385, PDB-9ngi: ELIC5 with propylamine facing ECD inwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 4.3 Å 49390 9ngq EMDB-49390, PDB-9ngq: ELIC state 1 with propylamine facing ECD outwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 3.8 Å 49391 9ngr EMDB-49391, PDB-9ngr: ELIC state 2 with propylamine facing ECD outwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 3.8 Å 49392 9ngs EMDB-49392, PDB-9ngs: ELIC with propylamine facing ECD inwards in liposomes with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 4.2 Å 49400 9nh4 EMDB-49400, PDB-9nh4: ELIC with propylamine in spNW25 nanodiscs with 2:1:1 POPC:POPE:POPG 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-3CN: 3-AMINOPROPANE ChemComp-NA: Unknown entry
由来	dickeya dadantii (バクテリア)
キーワード	MEMBRANE PROTEIN / ELIC / ion channel / pLGIC / Structural Protein / TRANSPORT PROTEIN