EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of folding intermediates on BAM show diverse substrates fold by a conserved mechanism.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 123, Issue 14, Page e2534936123, Year 2026
掲載日	2026年4月7日
著者	Benjamin D Thomson / Melissa D Marquez / Shaun Rawson / Thiago M A Dos Santos / Stephen C Harrison / Daniel Kahne /
PubMed 要旨	The outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria contain β-barrel membrane proteins that are assembled by conserved multisubunit machines. In bacteria, the β-barrel ...The outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria contain β-barrel membrane proteins that are assembled by conserved multisubunit machines. In bacteria, the β-barrel assembly machine (BAM) folds over a hundred compositionally different substrates into barrels that vary greatly in size. Some larger barrels require globular proteins to plug the barrel lumen. How a single machine can assemble such different barrels is unknown. Here we report three structures representing progressively folded stages of a 16-stranded barrel engaged with BAM, as well as the structure of a late-stage folding intermediate of a 26-stranded substrate folding around its soluble lipoprotein plug on BAM. We find that BAM catalyzes folding of these substrates by a uniform mechanism in which BAM undergoes major distortions to accommodate the nascent barrel.
リンク	Proc Natl Acad Sci U S A / PubMed:41926538 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 4.1 Å
構造データ	EMDB-48251: Consensus refinement of the barrel region of beta-barrel assembly machine from Escherichia coli in an late state of substrate assembly 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-48252: Focused refinement of the barrel region of beta-barrel assembly machine from Escherichia coli in an late state of substrate assembly 手法: EM (単粒子) / 解像度: 4.1 Å 48253 9mge EMDB-48253, PDB-9mge: beta-barrel assembly machine from Escherichia coli in an early state of substrate assembly 手法: EM (単粒子) / 解像度: 3.6 Å 48254 9mgf EMDB-48254, PDB-9mgf: beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly 手法: EM (単粒子) / 解像度: 3.3 Å 48255 9mgg EMDB-48255, PDB-9mgg: beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-71132: Consensus refinement of beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-71133: Focused refinement of beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly 手法: EM (単粒子) / 解像度: 4.1 Å 71134 9p1u EMDB-71134, PDB-9p1u: beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly 手法: EM (単粒子) / 解像度: 4.1 Å
由来	escherichia coli k-12 (大腸菌) escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN / beta-barrel assembly machine / outer membrane / folding intermediate