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-Structure paper
タイトル | The Architecture of Traveling Actin Waves Revealed by Cryo-Electron Tomography. |
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ジャーナル・号・ページ | Structure, Vol. 27, Issue 8, Page 1211-11223.e5, Year 2019 |
掲載日 | 2019年8月6日 |
![]() | Marion Jasnin / Florian Beck / Mary Ecke / Yoshiyuki Fukuda / Antonio Martinez-Sanchez / Wolfgang Baumeister / Günther Gerisch / ![]() |
PubMed 要旨 | Actin waves are dynamic supramolecular structures involved in cell migration, cytokinesis, adhesion, and neurogenesis. Although wave-like propagation of actin networks is a widespread phenomenon, the ...Actin waves are dynamic supramolecular structures involved in cell migration, cytokinesis, adhesion, and neurogenesis. Although wave-like propagation of actin networks is a widespread phenomenon, the actin architecture underlying wave propagation remained unknown. In situ cryo-electron tomography of Dictyostelium cells unveils the wave architecture and provides evidence for wave progression by de novo actin nucleation. Subtomogram averaging reveals the structure of Arp2/3 complex-mediated branch junctions in their native state, and enables quantitative analysis of the 3D organization of branching within the waves. We find an excess of branches directed toward the substrate-attached membrane, and tent-like structures at sites of branch clustering. Fluorescence imaging shows that Arp2/3 clusters follow accumulation of the elongation factor VASP. We propose that filament growth toward the membrane lifts up the actin network as the wave propagates, until depolymerization of oblique filaments at the back causes the collapse of horizontal filaments into a compact layer. |
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手法 | EM (サブトモグラム平均) |
解像度 | 26.0 - 32.0 Å |
構造データ | ![]() EMDB-4790: ![]() EMDB-4797: |
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