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-Structure paper
タイトル | Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism. |
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ジャーナル・号・ページ | Nat Commun, Vol. 10, Issue 1, Page 2754, Year 2019 |
掲載日 | 2019年6月21日 |
著者 | Valentin Mitterer / Ramtin Shayan / Sébastien Ferreira-Cerca / Guillaume Murat / Tanja Enne / Dana Rinaldi / Sarah Weigl / Hajrija Omanic / Pierre-Emmanuel Gleizes / Dieter Kressler / Celia Plisson-Chastang / Brigitte Pertschy / |
PubMed 要旨 | Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is ...Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps. |
リンク | Nat Commun / PubMed:31227701 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.15 - 3.8 Å |
構造データ | EMDB-4792, PDB-6rbd: EMDB-4793, PDB-6rbe: EMDB-4794: Yeast pre-40S Rps20 DeltaLoop C1-Headonly |
由来 |
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キーワード | RIBOSOME / pre-40S / yeast / cytoplasmic / mutant Rps20 |