EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural Insights into De Novo Promoter Escape by Mycobacterium tuberculosis RNA Polymerase.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 9990, Year 2025
掲載日	2025年11月13日
著者	Joshua Brewer / Madeleine Delbeau / Winston Bates Zoullas / Seth A Darst / Elizabeth A Campbell /
PubMed 要旨	Transcription in bacteria is a multi-step process. In the first step, contacts between RNA polymerase and the promoter DNA must be established for transcription initiation to begin, but then these ...Transcription in bacteria is a multi-step process. In the first step, contacts between RNA polymerase and the promoter DNA must be established for transcription initiation to begin, but then these contacts must be broken for the enzyme to transition into the elongation phase. Single-molecule and biochemical observations report that promoter escape is a highly regulated and sometimes rate-limiting step in the transcription cycle; however, the structural mechanisms of promoter escape remain obscure. Promoter escape also serves as the target for the clinically important antibiotic rifampicin, used to treat tuberculosis. Here, we present seven distinct intermediates showing the structural details of M. tuberculosis RNA polymerase initial transcribing complexes and promoter escape, using a de novo cryo-electron microscopy approach. We describe the structural rearrangements that RNA polymerase undergoes to clear the promoter, including those required to release the initiation factor, σ, providing a structural account for decades of biochemical observations. These structures and supporting biochemistry provide a model of promoter escape, a universal step in the transcription cycle, with conformations that may be used to develop Rifampicin alternatives.
リンク	Nat Commun / PubMed:41233305 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.2 Å
構造データ	47692 9e7v EMDB-47692, PDB-9e7v: Off-pathway Mycobacterium tuberculosis transcription initiation promoter complex (RNA Polymerase with Sigma-A, CarD, and RbpA) 手法: EM (単粒子) / 解像度: 3.4 Å 47695 9e7y EMDB-47695, PDB-9e7y: De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 7-mer RNA and disordered Beta' Lid element (RNA Polymerase with Sigma-A, CarD, and RbpA) 手法: EM (単粒子) / 解像度: 4.2 Å 47706 9e84 EMDB-47706, PDB-9e84: De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 6-mer RNA and disordered Sigma-A region 4 domain (RNA Polymerase with Sigma-A, CarD, and RbpA) 手法: EM (単粒子) / 解像度: 3.6 Å 47707 9e85 EMDB-47707, PDB-9e85: De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 6-mer RNA and closed Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA) 手法: EM (単粒子) / 解像度: 3.1 Å 47708 9e86 EMDB-47708, PDB-9e86: De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 5-mer RNA and open Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA) 手法: EM (単粒子) / 解像度: 3.8 Å 47709 9e87 EMDB-47709, PDB-9e87: De Novo Mycobacterium tuberculosis transcription initiation pre-RPO promoter complex with open Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA) 手法: EM (単粒子) / 解像度: 3.6 Å 47710 9e88 EMDB-47710, PDB-9e88: De novo backtracked transcription elongation complex of Mycobacterium tuberculosis RNA polymerase on a linear DNA fragment (TEC-Backtracked) 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-POP: PYROPHOSPHATE 2- ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry ChemComp-HOH: WATER
由来	mycobacterium tuberculosis (結核菌) synthetic construct (人工物)
キーワード	transcription/DNA / Promoter escape / transcription / transcription-DNA complex / TRANSCRIPTION/DNA/RNA / De novo / RNA polymerase / TRANSCRIPTION-DNA-RNA complex