EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.
ジャーナル・号・ページ	Cell, Vol. 187, Issue 9, Page 2236-2249.e17, Year 2024
掲載日	2024年4月25日
著者	Xian Xia / Po-Yu Sung / Michael W Martynowycz / Tamir Gonen / Polly Roy / Z Hong Zhou /
PubMed 要旨	Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques ...Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 Å, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond.
リンク	Cell / PubMed:38614100 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	2.8 - 7.2 Å
構造データ	43714 8w12 EMDB-43714, PDB-8w12: Cryo-EM structure of VP3-VP6 heterohexamer 手法: EM (単粒子) / 解像度: 3.5 Å 43716 8w19 EMDB-43716, PDB-8w19: Cryo-EM structure of BTV star-subcore 手法: EM (単粒子) / 解像度: 4.4 Å 43719 8w1c EMDB-43719, PDB-8w1c: Cryo-EM structure of BTV pre-subcore 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-43722: Cryo-EM structure of pre-subcore from in vitro assembled particles 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-43723: Cryo-EM structure of BTV empty virion 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-43724: Cryo-EM structure of BTV empty core 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-43725: Cryo-EM structure of BTV empty pre-core 手法: EM (単粒子) / 解像度: 3.2 Å 43726 8w1i EMDB-43726, PDB-8w1i: Cryo-EM structure of BTV subcore 手法: EM (単粒子) / 解像度: 6.5 Å 43727 8w1o EMDB-43727, PDB-8w1o: Cryo-EM structure of BTV virion 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-43728: Subtomogram averaging of BTV virion in host cells 手法: EM (サブトモグラム平均) / 解像度: 7.2 Å 43730 8w1r EMDB-43730, PDB-8w1r: Cryo-EM structure of BTV core 手法: EM (単粒子) / 解像度: 3.3 Å 43731 8w1s EMDB-43731, PDB-8w1s: Cryo-EM structure of BTV pre-core 手法: EM (単粒子) / 解像度: 3.1 Å
由来	bluetongue virus (serotype 1 / isolate south africa) (ウイルス)
キーワード	VIRAL PROTEIN / Bluetongue virus / capsid protein / heterohexamer / assembly intermediate / pre-subcore / virion / core