EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Atomic structures of a bacteriocin targeting Gram-positive bacteria.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 7057, Year 2024
掲載日	2024年8月16日
著者	Xiaoying Cai / Yao He / Iris Yu / Anthony Imani / Dean Scholl / Jeff F Miller / Z Hong Zhou /
PubMed 要旨	Due to envelope differences between Gram-positive and Gram-negative bacteria, engineering precision bactericidal contractile nanomachines requires atomic-level understanding of their structures; ...Due to envelope differences between Gram-positive and Gram-negative bacteria, engineering precision bactericidal contractile nanomachines requires atomic-level understanding of their structures; however, only those killing Gram-negative bacteria are currently known. Here, we report the atomic structures of an engineered diffocin, a contractile syringe-like molecular machine that kills the Gram-positive bacterium Clostridioides difficile. Captured in one pre-contraction and two post-contraction states, each structure fashions six proteins in the bacteria-targeting baseplate, two proteins in the energy-storing trunk, and a collar linking the sheath with the membrane-penetrating tube. Compared to contractile machines targeting Gram-negative bacteria, major differences reside in the baseplate and contraction magnitude, consistent with target envelope differences. The multifunctional hub-hydrolase protein connects the tube and baseplate and is positioned to degrade peptidoglycan during penetration. The full-length tape measure protein forms a coiled-coil helix bundle homotrimer spanning the entire diffocin. Our study offers mechanical insights and principles for designing potent protein-based precision antibiotics.
リンク	Nat Commun / PubMed:39152109 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 6.1 Å
構造データ	42953 8v3t EMDB-42953, PDB-8v3t: CryoEM Structure of Diffocin - precontracted - Collar 手法: EM (単粒子) / 解像度: 2.7 Å 42956 8v3w EMDB-42956, PDB-8v3w: CryoEM Structure of Diffocin - precontracted - Baseplate - focused refinement on triplex region 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-42957: CryoEM Structure of Diffocin - precontracted - Baseplate reconstructed in C6 symmetry 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-42958: CryoEM Structure of Diffocin - precontracted - Baseplate reconstructed in C3 symmetry 手法: EM (単粒子) / 解像度: 2.7 Å 42959 8v3x EMDB-42959, PDB-8v3x: CryoEM Structure of Diffocin - precontracted - Trunk 手法: EM (単粒子) / 解像度: 2.2 Å 42960 8v3y EMDB-42960, PDB-8v3y: CryoEM Structure of Diffocin - postcontracted - Trunk 手法: EM (単粒子) / 解像度: 3.6 Å 42961 8v3z EMDB-42961, PDB-8v3z: CryoEM Structure of Diffocin - postcontracted - Collar - transitional state 手法: EM (単粒子) / 解像度: 3.6 Å 42962 8v40 EMDB-42962, PDB-8v40: CryoEM Structure of Diffocin - postcontracted - Collar - final state 手法: EM (単粒子) / 解像度: 3.9 Å 42963 8v41 EMDB-42963, PDB-8v41: CryoEM Structure of Diffocin - postcontracted - Baseplate - transitional state 手法: EM (単粒子) / 解像度: 5.6 Å 42964 8v43 EMDB-42964, PDB-8v43: CryoEM Structure of Diffocin - postcontracted - Baseplate - final state 手法: EM (単粒子) / 解像度: 6.1 Å
由来	clostridioides difficile (バクテリア)
キーワード	VIRUS LIKE PARTICLE / Phage tail-like / bacteriocin / collar / pre-contraction / baseplate / trunk / post-contraction