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-Structure paper
タイトル | Photoreception and signaling in bacterial phytochrome revealed by single-particle cryo-EM. |
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ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 32, Page eadq0653, Year 2024 |
掲載日 | 2024年8月9日 |
著者 | Tek Narsingh Malla / Carolina Hernandez / Srinivasan Muniyappan / David Menendez / Dorina Bizhga / Joshua H Mendez / Peter Schwander / Emina A Stojković / Marius Schmidt / |
PubMed 要旨 | Phytochromes are red-light photoreceptors discovered in plants with homologs in bacteria and fungi that regulate a variety of physiological responses. They display a reversible photocycle between two ...Phytochromes are red-light photoreceptors discovered in plants with homologs in bacteria and fungi that regulate a variety of physiological responses. They display a reversible photocycle between two distinct states: a red-light-absorbing Pr state and a far-red light-absorbing Pfr state. The photoconversion regulates the activity of an enzymatic domain, usually a histidine kinase (HK). The molecular mechanism that explains how light controls the HK activity is not understood because structures of unmodified bacterial phytochromes with HK activity are missing. Here, we report three cryo-electron microscopy structures of a wild-type bacterial phytochrome with HK activity determined as Pr and Pfr homodimers and as a Pr/Pfr heterodimer with individual subunits in distinct states. We propose that the Pr/Pfr heterodimer is a physiologically relevant signal transduction intermediate. Our results offer insight into the molecular mechanism that controls the enzymatic activity of the HK as part of a bacterial two-component system that perceives and transduces light signals. |
リンク | Sci Adv / PubMed:39121216 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.75 - 4.61 Å |
構造データ | EMDB-42448: Pr state of Stigmatella aurantiaca bacteriophytochrome 2 EMDB-42450, PDB-8upk: EMDB-42452, PDB-8upm: EMDB-42469, PDB-8uqi: EMDB-42472, PDB-8uqk: |
化合物 | ChemComp-BLA: |
由来 |
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キーワード | SIGNALING PROTEIN / Red light photoreceptor / two component system |