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-Structure paper
タイトル | Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography. |
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ジャーナル・号・ページ | Life Sci Alliance, Vol. 7, Issue 3, Year 2024 |
掲載日 | 2024年1月5日 |
著者 | Zhixian Zhang / Abigail R Moye / Feng He / Muyuan Chen / Melina A Agosto / Theodore G Wensel / |
PubMed 要旨 | Primary cilia mediate sensory signaling in multiple organisms and cell types but have structures adapted for specific roles. Structural defects in them lead to devastating diseases known as ...Primary cilia mediate sensory signaling in multiple organisms and cell types but have structures adapted for specific roles. Structural defects in them lead to devastating diseases known as ciliopathies in humans. Key to their functions are structures at their base: the basal body, the transition zone, the "Y-shaped links," and the "ciliary necklace." We have used cryo-electron tomography with subtomogram averaging and conventional transmission electron microscopy to elucidate the structures associated with the basal region of the "connecting cilia" of rod outer segments in mouse retina. The longitudinal variations in microtubule (MT) structures and the lumenal scaffold complexes connecting them have been determined, as well as membrane-associated transition zone structures: Y-shaped links connecting MT to the membrane, and ciliary beads connected to them that protrude from the cell surface and form a necklace-like structure. These results represent a clearer structural scaffold onto which molecules identified by genetics, proteomics, and superresolution fluorescence can be placed in our emerging model of photoreceptor sensory cilia. |
リンク | Life Sci Alliance / PubMed:38182160 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 40.0 - 80.0 Å |
構造データ | EMDB-41812: Subtomogram Averaging of the Triplet from Mouse Centrioles EMDB-41813: Subtomogram averaging of doublets with Y-links EMDB-41814: Subtomogram averaging of ciliary beads from ciliary membrane |
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