EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Immune memory shapes human polyclonal antibody responses to H2N2 vaccination.
ジャーナル・号・ページ	bioRxiv, Year 2023
掲載日	2023年8月24日
著者	Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Abigail M Jackson / Alesandra J Rodriguez / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita Chopde / Masaru Kanekiyo / Katherine V Houser / Grace L Chen / Adrian B McDermott / Sarah F Andrews / Andrew B Ward /
PubMed 要旨	Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase I clinical trial investigating a ferritin nanoparticle displaying H2 hemagglutinin in ...Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase I clinical trial investigating a ferritin nanoparticle displaying H2 hemagglutinin in H2-naïve and H2-exposed adults. Therefore, we could perform comprehensive structural and biochemical characterization of immune memory on the breadth and diversity of the polyclonal serum antibody response elicited after H2 vaccination. We temporally map the epitopes targeted by serum antibodies after first and second vaccinations and show previous H2 exposure results in higher responses to the variable head domain of hemagglutinin while initial responses in H2-naïve participants are dominated by antibodies targeting conserved epitopes. We use cryo-EM and monoclonal B cell isolation to describe the molecular details of cross-reactive antibodies targeting conserved epitopes on the hemagglutinin head including the receptor binding site and a new site of vulnerability deemed the medial junction. Our findings accentuate the impact of pre-existing influenza exposure on serum antibody responses.
リンク	bioRxiv / PubMed:37781590 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 22.8 Å
構造データ	41464 8tp2 EMDB-41464, PDB-8tp2: H2 hemagglutinin (A/Singapore/1/1957) in complex with RBS-targeting 1-1-1F05 手法: EM (単粒子) / 解像度: 3.1 Å 41465 8tp3 EMDB-41465, PDB-8tp3: H1 hemagglutinin (NC99) in complex with RBS-targeting Fab 1-1-1F05 手法: EM (単粒子) / 解像度: 3.6 Å 41466 8tp4 EMDB-41466, PDB-8tp4: H2 hemagglutinin (A/Singapore/1/1957) in complex with RBS-targeting Fab 1-1-1E04 手法: EM (単粒子) / 解像度: 3.3 Å 41467 8tp5 EMDB-41467, PDB-8tp5: H1 hemagglutinin (NC99) in complex with RBS-targeting Fab 1-1-1E04 手法: EM (単粒子) / 解像度: 2.9 Å 41468 8tp6 EMDB-41468, PDB-8tp6: H2 hemagglutinin (A/Singapore/1/1957) in complex with RBS-targeting Fab 4-1-1E02 手法: EM (単粒子) / 解像度: 3.1 Å 41469 8tp7 EMDB-41469, PDB-8tp7: H2 hemagglutinin (A/Singapore/1/1957) in complex with Sa-targeting Fab 4-1-1G03 手法: EM (単粒子) / 解像度: 2.8 Å 41470 8tp9 EMDB-41470, PDB-8tp9: H2 hemagglutinin (A/Singapore/1/1957) in complex with medial-junction-targeting Fab 2-2-1G06 手法: EM (単粒子) / 解像度: 3.1 Å 41471 8tpa EMDB-41471, PDB-8tpa: H1 hemagglutinin (NC99) in complex with medial-junction-targeting Fab 2-2-1G06 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-41472: H2 hemagglutinin (A/Singapore/1/1957) in complex with polyclonal Fab, pAb_1 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-41473: H2 hemagglutinin (A/Singapore/1/1957) in complex with polyclonal Fab, pAb_2 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-41474: H2 hemagglutinin (A/Singapore/1/1957) in complex with polyclonal Fab, pAb_3 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-41523: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-1 at week 4 手法: EM (単粒子) / 解像度: 22.8 Å EMDB-41524: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-1 at week 16 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41525: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-1 at week 20 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41526: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-2 at week 0 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41527: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-2 at week 4 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41528: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-2 week 16 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41529: Polyclonal immune complex of Fab binding to H2 HA from serum of subject 2-2 at week 20 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41530: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 2-3 at week 4 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41531: Polyclonal immune complex of Fab binding H2 HA from serum of subject 2-3 at week 16 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41683: H2 HA in complex with Cb-targeting mAb 2-2-1E08 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41684: H2 HA in complex with esterase-targeting mAb 2-2-1C06 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41685: H2 HA in complex with RBS-targeting mAb 4-1-1E02 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41686: H2 HA in complex with Sa-targeting mAb 4-1-1G03 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41687: H2 HA in complex with Sa/Sb-targeting mAb 1-3-1F08 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41688: H2 HA in complex with RBS-targeting mAb 1-1-1F05 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41689: H2 HA in complex with Sa/Sb-targeting mAb 2-2-1F01 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41690: H2 HA in complex with medial-junction-targeting mAb 2-2-1G06 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41691: H2 HA in complex with RBS-targeting mAb 1-2-189-34 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41692: H2 HA in complex with RBS-targeting mAb 1-1-1A09 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41693: H2 HA in complex with RBS-targeting mAb 1-1-2A11 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-41694: H2 HA in complex with Sb-targeting mAb 1-1-2E05 手法: EM (単粒子) / 解像度: 20.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	influenza a virus (a/singapore/1/1957(h2n2)) (A型インフルエンザウイルス) homo sapiens (ヒト) influenza a virus (a/new caledonia/20/1999(h1n1)) (A型インフルエンザウイルス)
キーワード	VIRAL PROTEIN/Immune System / influenza / hemagglutinin / monoclonal antibody / immune complex / VIRAL PROTEIN / VIRAL PROTEIN-Immune System complex