EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the - respiratory supercomplex from .
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 120, Issue 40, Page e2307093120, Year 2023
掲載日	2023年10月3日
著者	Justin M Di Trani / Andreea A Gheorghita / Madison Turner / Peter Brzezinski / Pia Ädelroth / Siavash Vahidi / P Lynne Howell / John L Rubinstein /
PubMed 要旨	Energy conversion by electron transport chains occurs through the sequential transfer of electrons between protein complexes and intermediate electron carriers, creating the proton motive force that ...Energy conversion by electron transport chains occurs through the sequential transfer of electrons between protein complexes and intermediate electron carriers, creating the proton motive force that enables ATP synthesis and membrane transport. These protein complexes can also form higher order assemblies known as respiratory supercomplexes (SCs). The electron transport chain of the opportunistic pathogen is closely linked with its ability to invade host tissue, tolerate harsh conditions, and resist antibiotics but is poorly characterized. Here, we determine the structure of a SC that forms between the quinol:cytochrome oxidoreductase (cytochrome ) and one of the organism's terminal oxidases, cytochrome , which is found only in some bacteria. Remarkably, the SC structure also includes two intermediate electron carriers: a diheme cytochrome and a single heme cytochrome . Together, these proteins allow electron transfer from ubiquinol in cytochrome to oxygen in cytochrome . We also present evidence that different isoforms of cytochrome can participate in formation of this SC without changing the overall SC architecture. Incorporating these different subunit isoforms into the SC would allow the bacterium to adapt to different environmental conditions. Bioinformatic analysis focusing on structural motifs in the SC suggests that cytochrome - SCs also exist in other bacterial pathogens.
リンク	Proc Natl Acad Sci U S A / PubMed:37751552 / PubMed Central
手法	EM (単粒子)
解像度	2.3 - 3.0 Å
構造データ	40601 8smr EMDB-40601, PDB-8smr: cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa 手法: EM (単粒子) / 解像度: 2.7 Å 40625 8snh EMDB-40625, PDB-8snh: cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-40626: cytochrome bc1 complex from Pseudomonas aeruginosa 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-40627: cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa (Locally refined cytochrome cbb3) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-40637: cytochrome bc1 complex from Pseudomonas aeruginosa (Locally refined bc1 monomer with Rieske head domain in b state) 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-40638: cytochrome bc1 complex from Pseudomonas aeruginosa (Locally refined bc1 monomer with Rieske head domain in c state) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-40643: cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa (Locally refined cytochrome cbb3 with CcoP1 isoform) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-40645: cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa (Locally refined cytochrome cbb3 with CcoP2 isoform) 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-U10: UBIQUINONE-10 / 2-(3,7,11,15,19,23,27,31,35,39-デカメチルテトラコンタ-2,6,10,14,18,22,(以下略) ChemComp-I7Y: (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside ChemComp-HEC: HEME C ChemComp-CU: COPPER (II) ION ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	pseudomonas aeruginosa (緑膿菌)
キーワード	MEMBRANE PROTEIN / Electron transport chain / Supercomplex / Pseudomonas aeruginosa