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-Structure paper
タイトル | Evolution of higher torque in Campylobacter-type bacterial flagellar motors. |
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ジャーナル・号・ページ | Sci Rep, Vol. 8, Issue 1, Page 97, Year 2018 |
掲載日 | 2018年1月8日 |
![]() | Bonnie Chaban / Izaak Coleman / Morgan Beeby / ![]() ![]() |
PubMed 要旨 | Understanding the evolution of molecular machines underpins our understanding of the development of life on earth. A well-studied case are bacterial flagellar motors that spin helical propellers for ...Understanding the evolution of molecular machines underpins our understanding of the development of life on earth. A well-studied case are bacterial flagellar motors that spin helical propellers for bacterial motility. Diverse motors produce different torques, but how this diversity evolved remains unknown. To gain insights into evolution of the high-torque ε-proteobacterial motor exemplified by the Campylobacter jejuni motor, we inferred ancestral states by combining phylogenetics, electron cryotomography, and motility assays to characterize motors from Wolinella succinogenes, Arcobacter butzleri and Bdellovibrio bacteriovorus. Observation of ~12 stator complexes in many proteobacteria, yet ~17 in ε-proteobacteria suggest a "quantum leap" evolutionary event. Campylobacter-type motors have high stator occupancy in wider rings of additional stator complexes that are scaffolded by large proteinaceous periplasmic rings. We propose a model for motor evolution wherein independent inner- and outer-membrane structures fused to form a scaffold for additional stator complexes. Significantly, inner- and outer-membrane associated structures have evolved independently multiple times, suggesting that evolution of such structures is facile and poised the ε-proteobacteria to fuse them to form the high-torque Campylobacter-type motor. |
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手法 | EM (サブトモグラム平均) |
解像度 | 82.0 - 98.0 Å |
構造データ | ![]() EMDB-3910: ![]() EMDB-3911: ![]() EMDB-3912: |
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