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-Structure paper
タイトル | NMR analysis of a loop-bulge structure of UUCGA pentaloop. |
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ジャーナル・号・ページ | Biochem Biophys Res Commun, Vol. 691, Page 149327, Year 2024 |
掲載日 | 2024年1月8日 |
著者 | Rika Ichijo / Gota Kawai / |
PubMed 要旨 | Although structures of many RNA loops, such as GNRA and UNCG tetraloops, were well known, it is still possible to find more RNA structures. In the present study, solution structure of an RNA fragment ...Although structures of many RNA loops, such as GNRA and UNCG tetraloops, were well known, it is still possible to find more RNA structures. In the present study, solution structure of an RNA fragment having UUCGA pentaloop was analyzed by NMR spectroscopy. It was found that the UUCG tetraloop is formed and the adenosine residue at the 3' side of the tetraloop is bulged out. The characteristic motif of the loop-bulge structure has also been found in other RNAs including CUUGU and CUGGC pentaloops. Along with the recently found T-hairpin structure with a UUUGAUU loop, in which UUUGA pentaloop and UU bulge are formed, the loop-bulge structures can be categorized as an RNA motif and it may be called as the integrated structure loop, I-loop. |
リンク | Biochem Biophys Res Commun / PubMed:38039839 |
手法 | EM (単粒子) / NMR (溶液) |
解像度 | 3.97 Å |
構造データ | EMDB-39001, PDB-8y6u: PDB-8k8b: |
由来 |
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キーワード | RNA / penta loop / UUCG loop / TRANSCRIPTION/DNA / RNA polymerase / TRANSCRIPTION-DNA COMPLEX |