EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Spike N354 glycosylation augments SARS-CoV-2 fitness for human adaptation through structural plasticity.
ジャーナル・号・ページ	Natl Sci Rev, Vol. 11, Issue 7, Page nwae206, Year 2024
掲載日	2024年6月14日
著者	Pan Liu / Can Yue / Bo Meng / Tianhe Xiao / Sijie Yang / Shuo Liu / Fanchong Jian / Qianhui Zhu / Yuanling Yu / Yanyan Ren / Peng Wang / Yixin Li / Jinyue Wang / Xin Mao / Fei Shao / Youchun Wang / Ravindra Kumar Gupta / Yunlong Cao / Xiangxi Wang /
PubMed 要旨	Selective pressures have given rise to a number of SARS-CoV-2 variants during the prolonged course of the COVID-19 pandemic. Recently evolved variants differ from ancestors in additional ...Selective pressures have given rise to a number of SARS-CoV-2 variants during the prolonged course of the COVID-19 pandemic. Recently evolved variants differ from ancestors in additional glycosylation within the spike protein receptor-binding domain (RBD). Details of how the acquisition of glycosylation impacts viral fitness and human adaptation are not clearly understood. Here, we dissected the role of N354-linked glycosylation, acquired by BA.2.86 sub-lineages, as a RBD conformational control element in attenuating viral infectivity. The reduced infectivity is recovered in the presence of heparin sulfate, which targets the 'N354 pocket' to ease restrictions of conformational transition resulting in a 'RBD-up' state, thereby conferring an adjustable infectivity. Furthermore, N354 glycosylation improved spike cleavage and cell-cell fusion, and in particular escaped one subset of ADCC antibodies. Together with reduced immunogenicity in hybrid immunity background, these indicate a single spike amino acid glycosylation event provides selective advantage in humans through multiple mechanisms.
リンク	Natl Sci Rev / PubMed:39071099 / PubMed Central
手法	EM (単粒子)
解像度	3.18 - 3.93 Å
構造データ	37546 8whs EMDB-37546, PDB-8whs: Spike Trimer of BA.2.86 in complex with one hACE2 手法: EM (単粒子) / 解像度: 3.33 Å 37548 8whu EMDB-37548, PDB-8whu: Spike Trimer of BA.2.86 in complex with two hACE2s 手法: EM (単粒子) / 解像度: 3.3 Å 37549 8whv EMDB-37549, PDB-8whv: Spike Trimer of BA.2.86 with three RBDs down 手法: EM (単粒子) / 解像度: 3.32 Å 37550 8whw EMDB-37550, PDB-8whw: Spike Trimer of BA.2.86 with single RBD up 手法: EM (単粒子) / 解像度: 3.85 Å 37553 8whz EMDB-37553, PDB-8whz: BA.2.86 RBD in complex with hACE2 (local refinement) 手法: EM (単粒子) / 解像度: 3.93 Å 38049 8x4h EMDB-38049, PDB-8x4h: SARS-CoV-2 JN.1 Spike 手法: EM (単粒子) / 解像度: 3.65 Å 38056 8x4z EMDB-38056, PDB-8x4z: BA.2.86 Spike Trimer with ins483V mutation (3 RBD down) 手法: EM (単粒子) / 解像度: 3.49 Å 38057 8x50 EMDB-38057, PDB-8x50: BA.2.86 Spike Trimer with ins483V mutation (1 RBD up) 手法: EM (単粒子) / 解像度: 3.82 Å 38063 8x55 EMDB-38063, PDB-8x55: BA.2.86 Spike Trimer with T356K mutation (3 RBD down) 手法: EM (単粒子) / 解像度: 3.75 Å 38064 8x56 EMDB-38064, PDB-8x56: BA.2.86 Spike Trimer with T356K mutation (1 RBD up) 手法: EM (単粒子) / 解像度: 3.93 Å 38072 8x5q EMDB-38072, PDB-8x5q: SARS-CoV-2 BA.2.75 Spike with K356T mutation (3 RBD down) 手法: EM (単粒子) / 解像度: 3.47 Å 38073 8x5r EMDB-38073, PDB-8x5r: SARS-CoV-2 BA.2.75 Spike with K356T mutation (1 RBD up) 手法: EM (単粒子) / 解像度: 3.72 Å 38681 8xur EMDB-38681, PDB-8xur: BA.2.86 Spike Trimer in complex with heparan sulfate 手法: EM (単粒子) / 解像度: 3.85 Å 38682 8xus EMDB-38682, PDB-8xus: JN.1 Spike Trimer in complex with heparan sulfate 手法: EM (単粒子) / 解像度: 3.18 Å 38683 8xut EMDB-38683, PDB-8xut: XBB.1.5 Spike Trimer in complex with heparan sulfate 手法: EM (単粒子) / 解像度: 3.2 Å 38684 8xuu EMDB-38684, PDB-8xuu: BA.2.86-T356K Spike Trimer in complex with heparan sulfate (Local refinement) 手法: EM (単粒子) / 解像度: 3.69 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-ZN: Unknown entry ChemComp-CL: Unknown entry / クロリド ChemComp-IDU: 2-O-sulfo-beta-L-altropyranuronic acid
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/HYDROLASE / surface protein / glycoprotein / spike protein / VIRAL PROTEIN-HYDROLASE complex / VIRAL PROTEIN / Timer / Trimer / Complex / Spike / SARS-CoV-2