EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transport mechanism of presynaptic high-affinity choline uptake by CHT1.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 4, Page 701-709, Year 2024
掲載日	2024年4月8日
著者	Yunlong Qiu / Yiwei Gao / Bo Huang / Qinru Bai / Yan Zhao /
PubMed 要旨	Choline is a vital nutrient and a precursor for the biosynthesis of essential metabolites, including acetylcholine (ACh), that play a central role in fetal development, especially in the brain. In ...Choline is a vital nutrient and a precursor for the biosynthesis of essential metabolites, including acetylcholine (ACh), that play a central role in fetal development, especially in the brain. In cholinergic neurons, the high-affinity choline transporter (CHT1) provides an extraordinarily efficient reuptake mechanism to reutilize choline derived from intrasynaptical ACh hydrolysis and maintain ACh synthesis in the presynapse. Here, we determined structures of human CHT1 in three discrete states: the outward-facing state bound with the competitive inhibitor hemicholinium-3 (HC-3); the inward-facing occluded state bound with the substrate choline; and the inward-facing apo open state. Our structures and functional characterizations elucidate how the inhibitor and substrate are recognized. Moreover, our findings shed light on conformational changes when transitioning from an outward-facing to an inward-facing state and establish a framework for understanding the transport cycle, which relies on the stabilization of the outward-facing state by a short intracellular helix, IH1.
リンク	Nat Struct Mol Biol / PubMed:38589607
手法	EM (単粒子)
解像度	3.6 - 3.7 Å
構造データ	36025 8j74 EMDB-36025, PDB-8j74: Human high-affinity choline transporter CHT1 in the HC-3-bound outward-facing open conformation, dimeric state 手法: EM (単粒子) / 解像度: 3.6 Å 36027 8j75 EMDB-36027, PDB-8j75: Human high-affinity choline transporter CHT1 in the HC-3-bound outward-facing open conformation, monomeric state 手法: EM (単粒子) / 解像度: 3.6 Å 36029 8j76 EMDB-36029, PDB-8j76: Human high-affinity choline transporter CHT1 in the inward-facing apo-open conformation 手法: EM (単粒子) / 解像度: 3.7 Å 36030 8j77 EMDB-36030, PDB-8j77: Human high-affinity choline transporter CHT1 in the choline-bound inward-facing occluded conformation 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト ChemComp-AV0: Lauryl Maltose Neopentyl Glycol / ラウリルマルト-スネオペンチルグリコ-ル ChemComp-R16: HEXADECANE / ヘキサデカン / ヘキサデカン ChemComp-HC6: (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium) ChemComp-CHT: CHOLINE ION / コリン / コリン (栄養素)
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / CHT1 / SLC5A7 / high affinity choline transporter / choline transporter