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-Structure paper
タイトル | Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state. |
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ジャーナル・号・ページ | Science, Vol. 355, Issue 6330, Page 1181-1184, Year 2017 |
掲載日 | 2017年3月17日 |
![]() | Joost Snijder / Jan M Schuller / Anika Wiegard / Philip Lössl / Nicolas Schmelling / Ilka M Axmann / Jürgen M Plitzko / Friedrich Förster / Albert J R Heck / ![]() ![]() |
PubMed 要旨 | Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of ...Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator. |
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手法 | EM (単粒子) |
解像度 | 4.7 - 7.0 Å |
構造データ | EMDB-3602, PDB-5n8y: ![]() EMDB-3603: |
由来 |
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![]() | TRANSFERASE / AAA+-ATPase / Kinase / Circadian Clock Complex / Cyanobacteria / Fold-switch |