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-Structure paper
| タイトル | In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 1311, Year 2024 |
| 掲載日 | 2024年2月12日 |
 著者 | Weimin Li / Angdi Li / Bing Yu / Xiaoxiao Zhang / Xiaoyan Liu / Kate L White / Raymond C Stevens / Wolfgang Baumeister / Andrej Sali / Marion Jasnin / Liping Sun /    |
| PubMed 要旨 | Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and ...Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated insulin secretion at nanoscale resolution. After remodeling, the actin filament network at the cell periphery exhibits three marked differences: 12% of actin filaments reorient quasi-orthogonally to the ventral membrane; the filament network mainly remains as cell-stabilizing bundles but partially reconfigures into a less compact arrangement; actin filaments anchored to the ventral membrane reorganize from a "netlike" to a "blooming" architecture. Furthermore, the density of actin filaments and microtubules around insulin secretory granules decreases, while actin filaments and microtubules become more densely packed. The actin filament network after remodeling potentially precedes the transport and release of insulin secretory granules. These findings advance our understanding of actin remodeling and its role in glucose-stimulated insulin secretion. |
 リンク | Nat Commun / PubMed:38346988 / PubMed Central |
| 手法 | EM (トモグラフィー) |
| 構造データ |  EMDB-35809: Cellular components in INS-1E cell periphery  EMDB-35839: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35840: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35841: Cellular components at INS-1E cell periphery under basal condition  EMDB-35842: Cellular components at INS-1E cell periphery under basal condition  EMDB-35843: Cellular components at INS-1E cell periphery under basal condition  EMDB-35844: Cellular components at INS-1E cell periphery under basal condition  EMDB-35845: Cellular components at INS-1E cell periphery under basal condition  EMDB-35846: Cellular components at INS-1E cell periphery under basal condition  EMDB-35847: Cellular components at INS-1E cell periphery under basal condition  EMDB-35848: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35849: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35850: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35851: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35852: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35853: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35854: Cellular components at INS-1E cell periphery under second phase of glucose-stimulated insulin secretion  EMDB-35855: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35856: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35857: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35858: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35859: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35860: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35861: Cellular components at INS-1E cell periphery under first phase of glucose-stimulated insulin secretion  EMDB-35874: Cellular components at INS-1E cell interior under basal condition  EMDB-35875: Cellular components at INS-1E cell interior under basal condition  EMDB-35876: Cellular components at INS-1E cell interior under basal condition  EMDB-35885: Cellular components at INS-1E cell interior under basal condition  EMDB-35886: Cellular components at INS-1E cell interior under first phase of glucose-stimulated insulin secretion  EMDB-35887: Cellular components at INS-1E cell interior under first phase of glucose-stimulated insulin secretion  EMDB-35889: Cellular components at INS-1E cell interior under first phase of glucose-stimulated insulin secretion  EMDB-35890: Cellular components at INS-1E cell interior under first phase of glucose-stimulated insulin secretion  EMDB-35891: Cellular components at INS-1E cell interior under second phase of glucose-stimulated insulin secretion  EMDB-35892: Cellular components at INS-1E cell interior under second phase of glucose-stimulated insulin secretion  EMDB-35893: Cellular components at INS-1E cell interior under second phase of glucose-stimulated insulin secretion  EMDB-35894: Cellular components at INS-1E cell interior under second phase of glucose-stimulated insulin secretion  EMDB-35895: Cellular components at INS-1E cell interior under second phase of glucose-stimulated insulin secretion  EMDB-35896: Cellular components at INS-1E cell interior under basal condition  EMDB-35897: Cellular components at INS-1E cell interior under first phase of glucose-stimulated insulin secretion  EMDB-35935: Cellular components at INS-1E cell interior under basal condition  EMDB-35936: Cellular components at INS-1E cell interior under first phase of glucose-stimulated insulin secretion  EMDB-35937: Cellular components at INS-1E cell interior under second phase of glucose-stimulated insulin secretion  EMDB-37278: Cellular components at rat primary beta-cell periphery under second phase  EMDB-37279: Cellular components at rat primary beta-cell periphery under second phase  EMDB-37280: Cellular components at rat primary beta-cell periphery under second phase  EMDB-37281: Cellular components at rat primary beta-cell periphery under basal condition  EMDB-37282: Cellular components at rat primary beta-cell periphery under basal condition  EMDB-37283: Cellular components at rat primary beta-cell periphery under basal condition  EMDB-37284: Cellular components at rat primary beta-cell periphery under basal condition  EMDB-37285: Cellular components at rat primary beta-cell periphery under second phase |
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Rattus (ネズミ)