EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria.
ジャーナル・号・ページ	Nat Metab, Vol. 5, Issue 7, Page 1111-1126, Year 2023
掲載日	2023年6月22日
著者	Kuan-Jen Lu / Chiung-Wen Chang / Chun-Hsiung Wang / Frederic Y-H Chen / Irene Y Huang / Pin-Hsuan Huang / Cheng-Han Yang / Hsiang-Yi Wu / Wen-Jin Wu / Kai-Cheng Hsu / Meng-Chiao Ho / Ming-Daw Tsai / James C Liao /
PubMed 要旨	Regulation of CO fixation in cyanobacteria is important both for the organism and global carbon balance. Here we show that phosphoketolase in Synechococcus elongatus PCC7942 (SeXPK) possesses a ...Regulation of CO fixation in cyanobacteria is important both for the organism and global carbon balance. Here we show that phosphoketolase in Synechococcus elongatus PCC7942 (SeXPK) possesses a distinct ATP-sensing mechanism, where a drop in ATP level allows SeXPK to divert precursors of the RuBisCO substrate away from the Calvin-Benson-Bassham cycle. Deleting the SeXPK gene increased CO fixation particularly during light-dark transitions. In high-density cultures, the Δxpk strain showed a 60% increase in carbon fixation and unexpectedly resulted in sucrose secretion without any pathway engineering. Using cryo-EM analysis, we discovered that these functions were enabled by a unique allosteric regulatory site involving two subunits jointly binding two ATP, which constantly suppresses the activity of SeXPK until the ATP level drops. This magnesium-independent ATP allosteric site is present in many species across all three domains of life, where it may also play important regulatory functions.
リンク	Nat Metab / PubMed:37349485 / PubMed Central
手法	EM (単粒子)
解像度	2.17 - 2.86 Å
構造データ	35609 8io6 EMDB-35609, PDB-8io6: Cryo-EM structure of phosphoketolase from Bifidobacterium longum in octameric assembly 手法: EM (単粒子) / 解像度: 2.68 Å 35610 8io7 EMDB-35610, PDB-8io7: Cryo-EM structure of phosphoketolase from Bifidobacterium longum in dimeric assembly 手法: EM (単粒子) / 解像度: 2.62 Å 35611 8io8 EMDB-35611: Cryo-EM structure of cyanobacteria phosphoketolase complexed with AMPPNP in dimeric assembly PDB-8io8: Cryo-EM structure of cyanobacteria phosphoketolase complexed with AMPPNPin dimeric assembly 手法: EM (単粒子) / 解像度: 2.17 Å 35612 8io9 EMDB-35612: Cryo-EM structure of cyanobacteria phosphoketolase complexed with AMPPNPin dodecameric assembly PDB-8io9: Cryo-EM structure of cyanobacteria phosphoketolase complexed with AMPPNP in dodecameric assembly 手法: EM (単粒子) / 解像度: 2.36 Å 35613 8ioa EMDB-35613, PDB-8ioa: Cryo-EM structure of cyanobacteria phosphoketolase 手法: EM (単粒子) / 解像度: 2.63 Å 35617 8ioe EMDB-35617, PDB-8ioe: Cryo-EM structure of cyanobacteria phosphoketolase in dodecameric assembly 手法: EM (単粒子) / 解像度: 2.86 Å
化合物	ChemComp-TPP: THIAMINE DIPHOSPHATE / 3-[(4-アミノ-2-メチル-5-ピリミジニル)メチル]-4-メチル-5-[2-(ホスホノオキシホスフィナトオキシ)(以下略) ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	Bifidobacteriaceae bacterium (バクテリア) bifidobacterium longum subsp. longum f8 (バクテリア) Synechococcus (バクテリア) synechococcus elongatus (strain atcc 33912 / pcc 7942 / fachb-805) (バクテリア)
キーワード	CYTOSOLIC PROTEIN / Carbon metabolism / TPP-dependent enzyme